The visible 14-kDa band was excised out with razor blade and was subjected to protein sequencing
5uC for 10 s, 60uC for 30 s; 72uC PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19718802 immunoprecipitates were washed four times with RIPA buffer and the immunoprecipitated proteins were analyzed by western blot using ubiqutin…