Horseradish peroxidase-conjugated goat anti-mouse (Amersham Biosciences) secondary antibody was used at a dilution of 1:2000. The blots were processed with ECL Chemiluminescence kit (Amersham Biosciences), and the sign was detected by exposing the processed blots to X-ray movies (Biomax Movies, Kodak, NY)
Hence, the combination of anti-TR and anti-non TR MUC4 antibodies can give better information about the extent of MUC4 overexpression in the tumor tissues. Efforts are underway to study the…