Product Name: Astrocytes, GFAP Nanoluc-Halotag KI Line
Product Type: Chemical
CAS NO: 310456-65-6HIV inhibitors
biological source
from human
growth mode
Adherent
karyotype
2n=46
morphology
elongated, flat, polarized, astrocytic
Shipped in: dry ice
Storage temp.: −196°C
Application: These products provide unique and versatile tools for the study of central nervous system (CNS) disorders, such as Alzheimer’s disease (AD), Parkinson’s disease (PD), autism, schizophrenia, and amyotrophic lateral sclerosis (ALS), as well as for drug screening and toxicology applications in the neural space.
Cell Line Description:
monoallelic knock-in of GFAP nanoluc-halotag reporter
Culture Medium:
User must also purchase Astrocyte Maturation Medium, AST1050 (50mL) or AST1051 (250mL). Sold separately.
Features and Benefits:
This product contains cryo-preserved, pre-differentiated astrocytes with a GFAP Nanoluc-Halotag reporter knocked-in with zinc fingers. They were derived from a footprint-free, karyotype normal human iPSC line. It was designed for customers to generate mature astrocytes using the optimized maturation medium and supplements (AST1050, AST1051 sold separately). Mature astrocytes can be obtained within 17 days. Shortly after seeding and recovery, the cells proliferate and need to be passaged a minimum of two times. In general, at 17 days post-seeding, the cell population will contain ≥80% Glial Fibrillary Acidic Protein (GFAP) positive astrocytes and ≤15% Tuj-1 positive neurons (neuronal class III β-tubulin).
Subculture Routine:
The cells need to be passaged a minimum of two times. See technical bulletin for additional details.
RIDADR: NONH for all modes of transport
WGK Germany: 3
Storage Temp.: −196°C
UNSPSC
12352200
PubMed ID:http://jpet.aspetjournals.org/content/327/2/592