Name :
Recombinant Human PD-1 Protein (ECD, His Tag), HPLC-verified
Biological Activity :
Background :
Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Blockade: Blocking Antibodi Immune Checkpoint Blockade: PD1 / PDCD1 / CD2 Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Immunotherapy PD1 / PDCD1 / CD279 Immune Checkpoint PD1 / PDCD1 / CD279 Immune Checkpoint Antibod PD1 / PDCD1 / CD279 Immune Checkpoint Protein Targeted Therapy
Biological Activity :
1. Immobilized human PD-1 (Cat:10377-H08H) at 2 μg/ml (100 μl/well) can bind PD-L1 Protein, Human, Recombinant (ECD, hFc Tag) (Cat:10084-H02H), the EC50 of human B7-H1 is 150-600 ng/mL.2. Immobilized human PD-1 (Cat:10377-H08H) at 2 μg/mL (100 μL/well) can bind Anti-PD1(MDX)-IgG4 Antibody (Nivolumab)(Cat:68050-H001),the EC50 is 8-60 ng/mL.3. Loaded Anti-human PD1 antibody, IgG4 on ProA Biosensor, can bind Recombinant Human PD1 Protein, His Tag (Cat. NO. 10377-H08H) with an affinity constant of 2.44 nM as determined in a BLI assay (Sartorius Octet RED384) (Routinely tested).4.Captured Anti-PD1 Mab (Human IgG4) on proA Chip can bind PD1 (Cat.No.10377-H08H) with an affinity constant of 10.03nM as determined in a SPR assay (Biacore T200)(Routinely tested) .
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_005009.2
NCBI Gene ID :
Synonyms :
Synonyms :
programmed cell death 1
Amino Acid Sequence :
Molecular Weight :
The recombinant mature human PD1 consists of 154 amino acids and has a calculated molecular mass of 17.4 kDa. As a result of glycosylation, human PD1 migrates as an approximately 41.1 kDa protein in SDS-PAGE under reducing conditions.
Purity :
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the extracellular domain (Met 1-Gln 167) of human PD1 (NP_005009.2) was expressed with a C-terminal polyhistidine tag.
Buffer Solution :
Lyophilized from sterile 50mM MOPS, 500mM NaCl, pH 7.0Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CD279 Protein, Human; hPD-1 Protein, Human; hPD-l Protein, Human; hSLE1 Protein, Human; PD-1 Protein, Human; PD1 Protein, Human; SLEB2 Protein, Human PD-1 背景信息 Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Blockade: Blocking Antibodi Immune Checkpoint Blockade: PD1 / PDCD1 / CD2 Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Immunotherapy PD1 / PDCD1 / CD279 Immune Checkpoint PD1 / PDCD1 / CD279 Immune Checkpoint Antibod PD1 / PDCD1 / CD279 Immune Checkpoint Protein Targeted Therapy
References & Citations :
James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.
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