Name :
Recombinant Human PCSK9 Protein (D374Y, His Tag), HPLC-verified
Biological Activity :
Background :
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Biological Activity :
1.Measured by its binding ability in a functional ELISA. Immobilized Human LDLR mFc (Cat:10231-H05H) at 2 μg/ml (100 μl/well) can bind Human PCSK9(D374Y) His(Cat:29698-H08H1), the EC50 of PCSK9（D374Y) His is 1-20 ng/mL.2.Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag (Cat. No. 10231-H05H) on ProA Biosensor, can bind Recombinant Human PCSK9 (D374Y) Protein, His Tag (Cat. No. 29698-H08H1) with an affinity constant of 2.57nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_777596.2
NCBI Gene ID :
Synonyms :
Synonyms :
proprotein convertase subtilisin/kexin type 9
Amino Acid Sequence :
Molecular Weight :
The recombinant human PCSK9 consists of 673 amino acids and predicts a molecular mass of 72.5 kDa. As a result of glycosylation and proteolytic digestion, it migrates as doublet with apparent molecular mass of 19 kDa and 60 kDa corresponding to the pro domain and mature form respectively in SDS-PAGE under reducing conditions.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the human PCSK9 (NP_777596.2, with mutation Asp 374 Tyr) (Met1-Gln692) was expressed with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
FH3 Protein, Human; HCHOLA3 Protein, Human; LDLCQ1 Protein, Human; NARC-1 Protein, Human; NARC1 Protein, Human; PC9 Protein, Human PCSK9 背景信息 Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
References & Citations :
Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933. Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498 Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73. Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875. Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
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