Name :
Recombinant Human Optineurin Protein (His & GST Tag)
Biological Activity :
Background :
OPTN (Optineurin) is a Protein Coding gene. The Optineurin gene encodes the coiled-coil containing protein optineurin. Optineurin is a multifunctional adaptor protein intimately involved in various vesicular trafficking pathways. Through interactions with an array of proteins, such as myosin VI, huntingtin, Rab8, and Tank-binding kinase 1, as well as via its oligomerization, optineurin can act as an adaptor, scaffold, or signal regulator to coordinate many cellular processes associated with the trafficking of membrane-delivered cargo. It is widely expressed in fat, adrenal, and other tissues. Certain mutations in OPTN (gene OPTN) are associated with primary open-angle glaucoma, a leading cause of irreversible blindness, and amyotrophic lateral sclerosis, a fatal motor neuron disease.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
NP_068815.2
NCBI Gene ID :
Synonyms :
Synonyms :
optineurin
Amino Acid Sequence :
Molecular Weight :
The recombinant human OPTN chimera consists of 814 amino acids and has a calculated molecular mass of 93.7 kDa. The recombinant protein migrates approximately 94 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 85 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human OPTN (NP_068815.2) (Met1-Ile577) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
ALS12 Protein, Human; FIP2 Protein, Human; GLC1E Protein, Human; HIP7 Protein, Human; HYPL Protein, Human; NRP Protein, Human; TFIIIA-INTP Protein, Human Optineurin 背景信息 OPTN (Optineurin) is a Protein Coding gene. The Optineurin gene encodes the coiled-coil containing protein optineurin. Optineurin is a multifunctional adaptor protein intimately involved in various vesicular trafficking pathways. Through interactions with an array of proteins, such as myosin VI, huntingtin, Rab8, and Tank-binding kinase 1, as well as via its oligomerization, optineurin can act as an adaptor, scaffold, or signal regulator to coordinate many cellular processes associated with the trafficking of membrane-delivered cargo. It is widely expressed in fat, adrenal, and other tissues. Certain mutations in OPTN (gene OPTN) are associated with primary open-angle glaucoma, a leading cause of irreversible blindness, and amyotrophic lateral sclerosis, a fatal motor neuron disease.
References & Citations :
Rezaie T. et al., 2002, Science. 295 (5557): 1077-9. Li Y. et al., 1998, Mol Cell Biol. 18 (3): 1601-10. Skarnes Wc RB. et al., 2011, Nature. 474 (7351): 337-42.
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