Name :
Recombinant Human MAPKAPK3 Protein (GST Tag)
Biological Activity :
Background :
The MAPKAP kinases are a group of MAP kinase substrates that are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identified and serve as substrates for the ERK and p38 MAP kinases. Mitogen-activated protein (MAP) kinase-activated protein kinase 3, also known as MAPKAPK-3 and 3pK, is a member of the Ser/Thr protein kinase family. It is widely expressed in human tissues, with a higher expression level observed in the heart and skeletal muscle. No expression in the brain. MAPKAPK-3 is unique since it was shown to be activated by three members of the MAPK family, namely extracellular-signal-regulated kinase (ERK), p38, and Jun-N-terminal kinase (JNK). It is highly activated both by mitogens and by stress-inducing agents or proinflammatory cytokines and translocates to the cytoplasm from the nucleus. MAPKAPK-3 is exclusively activated via the classical MAPK cascade, while stress-induced activation of MAPKAPK-3 is mainly mediated by p38, however, the mechanism defining the specificity remains unknown.
Biological Activity :
Kinase activity untested
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
NP_004626.1
NCBI Gene ID :
Synonyms :
Synonyms :
mitogen-activated protein kinase-activated protein kinase 3
Amino Acid Sequence :
Molecular Weight :
The recombinant human MAPKAPK3/GST chimera consists of 607 amino acids and predicts a molecular mass of 69 kDa which is also estimated by SDS-PAGE.
Purity :
> 90 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the full length of human MAPKAPK3 (NP_004626.1) (Met 1-Gln 382) was expressed with the GST tag at the N-terminus.
Buffer Solution :
Supplied as sterile 50mM Tris, 100mM NaCl, pH 7.5, 0.25mM DTT, 0.1mM EDTA, 0.5mM PMSF, 10% glycerolPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Synonyms :
3PK Protein, Human; MAPKAP-K3 Protein, Human; MAPKAP3 Protein, Human; MAPKAPK-3 Protein, Human; MK-3 Protein, Human MAPKAPK3 背景信息 The MAPKAP kinases are a group of MAP kinase substrates that are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identified and serve as substrates for the ERK and p38 MAP kinases. Mitogen-activated protein (MAP) kinase-activated protein kinase 3, also known as MAPKAPK-3 and 3pK, is a member of the Ser/Thr protein kinase family. It is widely expressed in human tissues, with a higher expression level observed in the heart and skeletal muscle. No expression in the brain. MAPKAPK-3 is unique since it was shown to be activated by three members of the MAPK family, namely extracellular-signal-regulated kinase (ERK), p38, and Jun-N-terminal kinase (JNK). It is highly activated both by mitogens and by stress-inducing agents or proinflammatory cytokines and translocates to the cytoplasm from the nucleus. MAPKAPK-3 is exclusively activated via the classical MAPK cascade, while stress-induced activation of MAPKAPK-3 is mainly mediated by p38, however, the mechanism defining the specificity remains unknown.
References & Citations :
McLaughlin, M.M. et al., 1996, J. Biol. Chem. 271:8488-8492. Tanoue, T. et al., 2001, EMBO J. 20: 466-479. Neufeld, B. et al., 2000, J. Biol. Chem. 275: 20239-20242. Zakowski,V. et al., 2004, Exp Cell Res. 299: 101-109. Voncken, J. W. et al., 2005, J. Biol. Chem. 280: 5178-5187.
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