Name :
Recombinant Human Kallikrein 11 Protein (His Tag)
Biological Activity :
Background :
kallikrein-related peptidase 11 (KLK11), also known as hippostasin, trypsin-like serine protease, and PRSS2, is a member of the human tissue kallikrein family. It is a subgroup of serine proteases with diverse physiological functions, which are implicated in carcinogenesis and some with potential that serving as novel biomarkers for ovarian and prostate cancer and other diseases. The KLK11 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Two alternatively spliced forms exist, resulting in 25 (isoform 1) and 282 (isoform 2) amino acid sequences. Isoform 2 is identical to isoform 1, except for an inserted 32 amino acid segment. Isoform 1 is predominantly expressed in the brain whereas isoform 2 is preferentially expressed in the prostate.
Biological Activity :
Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB) (Edwards, K.M. et al.,1999, J. Biol. Chem. 274: 30468)
. The specific activity is >200 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_006844.1
NCBI Gene ID :
Synonyms :
Synonyms :
kallikrein-related peptidase 11
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant human KLKL11 comprises 243 amino acids with a predicted molecular mass of 27 kDa. As a result of glycosylation, rhKLK11 migrates as an approximately 40 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 90 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human KLK11 isoform 1 (NP_006844.1) (Met 1-Asn 250) with a C-terminal polyhistidine tag was expressed.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
KLK11 Protein, Human; PRSS20 Protein, Human; TLSP Protein, Human Kallikrein 11 背景信息 kallikrein-related peptidase 11 (KLK11), also known as hippostasin, trypsin-like serine protease, and PRSS2, is a member of the human tissue kallikrein family. It is a subgroup of serine proteases with diverse physiological functions, which are implicated in carcinogenesis and some with potential that serving as novel biomarkers for ovarian and prostate cancer and other diseases. The KLK11 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Two alternatively spliced forms exist, resulting in 25 (isoform 1) and 282 (isoform 2) amino acid sequences. Isoform 2 is identical to isoform 1, except for an inserted 32 amino acid segment. Isoform 1 is predominantly expressed in the brain whereas isoform 2 is preferentially expressed in the prostate.
References & Citations :
Yoshida S., et al.,(1998), cDNA cloning and expression of a novel serine protease, TLSP. Biochim. Biophys. Acta 1399:225-228.Mitsui S., et al., (2000), A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate.Biochem. Biophys. Res. Commun. 272:205-211.Yousef G.M., et al.,(2000), Genomic organization, mapping, tissue expression, and hormonal regulation of trypsin-like serine protease (TLSP PRSS20), a new member of the human kallikrein gene family.Genomics 63:88-96.
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