Name :
Human Integrin alpha 2b beta 3 Heterodimer Protein (I874S, Flag & His Tag)
Biological Activity :
Background :
Integrin alpha 2b beta 3 (also GPIIbIIIa) is a key platelet integrin, a noncovalent heterodimer of type I transmembrane subunits alpha 2b/CD41 and beta3/CD61. Capable of binding fibrinogen in its active state, its α and β subunits each comprise a ligand-binding extracellular domain, a single-pass transmembrane helix, and a cytoplasmic tail with binding sites for intracellular proteins. Upon agonist stimulation, αIIbβ3 accepts its extended-open (activated) conformation, increasing its matrix protein RGD motif ligand affinity, subsequently facilitating platelet spreading, platelet aggregation, and thrombus formation. Deficiencies lead to lifelong bleeding diathesis as seen in Glanzmann thrombasthenia. αIIbβ3-targeting therapeutic antibodies can inhibit thrombi formation in patients with acute coronary syndrome, and potentially inhibit tumor angiogenesis and metastasis by blocking interaction of platelet αIIbβ3 with tumor cells.
Biological Activity :
Immobilized Anti-GPIIb/IIIa Antibody (IgG1-Fab) at 2 μg/mL (100 μL/well) can bind Recombinant Human Integrin alpha 2b beta 3 Heterodimer Protein (Flag & His Tag) (Cat: CT131-H2508H), the EC50 is 18-54 ng/mL.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
P08514-1 & P05106-1
NCBI Gene ID :
Synonyms :
Synonyms :
Amino Acid Sequence :
Molecular Weight :
The recombinant Integrin alpha 2b beta 3 Heterodimer comprises 1738 (1004+734) amino acids and has a calculated molecular mass of 191.12 (109.68+81.44) KDa. The apparent molecular mass of Integrin alpha 2b beta 3 Heterodimer is approximately 115.6 KDa and 96.8 KDa respectively in SDS-PAGE.
Purity :
≥ 90 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the human ITGA2B (P08514-1)(Met1-Arg993, I874S) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human ITGB3 (P05106-1) (Met1-Asp718) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Integrin alpha 2b beta 3 Heterodimer was purified.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
1. Huang, J., et al. Platelet integrin αIIbβ3: signal transduction, regulation, and its therapeutic targeting. J Hematol Oncol, 2019. https://doi.org/10.1186/s13045-019-0709-62. Xin, H., et al. Structure, signal transduction, activation, and inhibition of integrin αIIbβ3. Thrombosis J, 2023. https://doi.org/10.1186/s12959-023-00463-w3. van den Kerkhof DL, et al. Exogenous Integrin αIIbβ3 Inhibitors Revisited: Past, Present and Future Applications. Int J Mol Sci, 2021. doi: 10.3390/ijms220733664. Coller BS. αIIbβ3: structure and function. J Thromb Haemost. 2015 Jun;13 Suppl 1(Suppl 1):S17-25. doi: 10.1111/jth.12915
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