Name :
Recombinant Human IL17A & IL17F Heterodimer Protein
Biological Activity :
Background :
Biological Activity :
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 20ng/mL TNFα. The ED50 for this effect is 1-6 ng/mL.
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
Q16552
NCBI Gene ID :
Synonyms :
Synonyms :
interleukin 17A
Amino Acid Sequence :
Molecular Weight :
The recombinant heterodimer of human IL17A & IL17F comprises 271 (137+134) amino acids and has a calculated molecular mass of 30.7 (15.7+15) kDa.
Purity :
> 95 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the human IL17A (Q16552) (Ile20-Ala155) was expressed, constructed the plasmid 1; the human IL17F (NP_443104.1) (Arg31-Gln163) was expressed, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CTLA8 Protein, Human; IL-17 Protein, Human; IL-17A Protein, Human; IL17 Protein, Human IL17A & IL17F Heterodimer 背景信息
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Fc gamma RIIA/CD32a ProteinMolecular Weight
ACE/CD143 Proteinmanufacturer
Popular categories:
IL-17RD
FGF-11