Name :
Recombinant Human IL-12 Protein (His Tag), GMP grade, HPLC-verified
Biological Activity :
Background :
Biological Activity :
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.04-0.4 ng/mL. The specific activity of recombinant human IL-12 is >10，000 Units/μg, which is calibrated against the human IL-12 WHO International Standard (NIBSC code: 95/544).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
P29459.2
NCBI Gene ID :
Synonyms :
Synonyms :
interleukin 12A
Amino Acid Sequence :
Molecular Weight :
The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.13 (23.99+36.14) kDa.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 5 EU per mg of the protein.
Protein Construction :
A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (P29459.2) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Buffer Solution :
Supplied as sterile PBS, pH 7.4Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Liquid. It is shipped out with blue ice.
Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Synonyms :
CLMF Protein, Human; CLMF2 Protein, Human; IL-12A Protein, Human; IL-12B Protein, Human; IL12 Protein, Human; IL12 p70 Protein, Human; IMD28 Protein, Human; IMD29 Protein, Human; NFSK Protein, Human; NKSF Protein, Human; NKSF1 Protein, Human; NKSF2 Protein, Human; P35 Protein, Human IL-12 背景信息
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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