Name :
Recombinant Human IGF1 Protein, HPLC-verified
Biological Activity :
Background :
IGF I, also known as Mechano Growth Factor, somatomedin-C, IGF-I, and IGF1, is a secreted protein that belongs to the insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II ( IGF-I and IGF-II ), and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also about enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.
Biological Activity :
1.Immobilized Recombinant Human IGF1 / IGF-I Protein (Cat: 10598-HNAE) at 2 μg/mL (100 μL/well) can bind Recombinant Human IGFBP4 Protein (His Tag) (Cat: 10967-H08H), the EC50 is 40-130 ng/mL.2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 3.5-14 ng/mL.3.Human breast cancer organoids were cultured with FGF7(Cat#10210-H07E), RSPO1(Cat#11083-HNAS), IGF1(Cat#10598-HNAE), EGF(Cat#50482-MNCH), NRG1 Beta 1(Cat#11609-H01H), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.4.Human breast organoids were cultured with IGF1(Cat#10598-HNAE), NRG1 Beta 1(Cat#11609-H01H). (Routinely tested). Data provided by D1 Medical Technology.5.Human ovarian organoids were cultured with IGF1(Cat#10598-HNAE), NRG1 Beta 1(Cat#11609-H01H), RSPO1(Cat#11083-HNAS), EGF(Cat#50482-MNCH), NOG(Cat#50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
P05019-1
NCBI Gene ID :
Synonyms :
Synonyms :
insulin-like growth factor 1 (somatomedin C)
Amino Acid Sequence :
Molecular Weight :
The recombinant human IGF1 consists of 71 amino acids and has a calculated molecular mass of 7.8 KDa.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
<10 EU per mg protein.
Protein Construction :
A DNA sequence encoding the human IGF1 (P05019-1) (Gly49-Ala118) was expressed.
Buffer Solution :
Lyophilized from sterile 30% Acetonitrile, 0.1% TFAPlease contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
IGF-1 Protein, Human; IGF-I Protein, Human; IGF1A Protein, Human; IGFI Protein, Human; IGF‑I Protein, Human; MGF Protein, Human IGF1 背景信息 IGF I, also known as Mechano Growth Factor, somatomedin-C, IGF-I, and IGF1, is a secreted protein that belongs to the insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II ( IGF-I and IGF-II ), and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also about enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.
References & Citations :
Jansen M., et al.,(1983), Sequence of cDNA encoding human insulin-like growth factor I precursor. Nature 306:609-611.de Pagter-Holthuizen P., et al., (1986), Organization of the human genes for insulin-like growth factors I and II.FEBS Lett. 195:179-184.le Bouc Y., et al.,(1986), Complete characterization of the human IGF-I nucleotide sequence isolated from a newly constructed adult liver cDNA library.FEBS Lett. 196:108-112.
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