Name :
Recombinant Chinese hamster PLBL2 / PLBD2 Protein (His Tag), HPLC-verified
Biological Activity :
Background :
PLBD2 localizes to the lysosome, as its absence could plausibly lead to a serious yet unrecognized lysosomal storage disease. PLBD1 and PLBD2 are semi-orphans in the sense of being probable phospholipases of B class but with uncertain physiological substrates and thus functionalities. PLBD1 and PLBD2 constitute a small gene family (sequence homology class) within vertebrates though one that occurs expanded in some early diverging eukaryotes. PLBD2 presents a special difficulty in that a sequence of post-translational steps are apparently necessary for its activation. Without these, potential substrates can hardly be assayed. These steps include removal of the signal peptide, mannosylation appropriate to the lysosome targeting receptor, and self-catalytic proteolytic activation to expose the substrate binding site as this becomes appropriate.
Biological Activity :
Testing in progress
Expression Host :
Chinese hamster
Source :
CHO Cells
Tag :
Protein Accession No. :
G3I6T1-1
NCBI Gene ID :
Synonyms :
Synonyms :
phospholipase B domain containing 2
Amino Acid Sequence :
Molecular Weight :
The recombinant chinese hamster PLBD2 consists of 559 amino acids and predicts a molecular mass of 63.3 kDa. It migrates as an approximately 65.3, 43.8 and 35.9 KDa band in SDS-PAGE under reducing conditions.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the chinese hamster PLBD2 (G3I6T1-1) (Leu38-Asp585) was expressed with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
Morgan CP. et al., 2004), Biochem J. 382 (2): 441-9. Kim W. et al., 2011, Mol Cell. 44 (2): 325-40. Havugimana PC. et al., 2012. Cell. 150 (5): 1068-81.
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