Name :
Recombinant Human HEXA Protein (Subunit A, His Tag)
Biological Activity :
Background :
Biological Activity :
Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MU-Glc-NAc).The specific activity is >1,250 pmoles/min/μg.
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
AAD13932.1
NCBI Gene ID :
Synonyms :
Synonyms :
hexosaminidase A (alpha polypeptide)
Amino Acid Sequence :
Molecular Weight :
The recombinant human HEXA consists of 517 amino acids and predicts a molecular mass of 59.7 kDa. It migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 95 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human HEXA (AAD13932.1) (Met 1-Thr 529) was fused with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
TSD Protein, Human HEXA 背景信息
References & Citations :
Norflus,F. et al., 1996, DNA Cell Biol. 15 (2):89-97. Kaufman M., et al., 1997, Hum. Mutat. 10:295-300. Tanaka A., et al., 2003, J. Hum. Genet. 48:571-574. Chen R., et al., 2009, J. Proteome Res. 8:651-661. Burkard T.R., et al., 2011, BMC Syst. Biol. 5:17-17.
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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