Name :
Recombinant Human Her2/ERBB2 Protein (ECD, His Tag), HPLC-verified
Biological Activity :
Background :
Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy
Biological Activity :
1. Immobilized Her2/ERBB2 Protein, Human, Recombinant (ECD, His Tag)(Cat:10004-H08H) at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab)(Cat:68046-H002), the EC50 is 10-60 ng/mL.2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.2-1 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.3.Captured anti-Her2 IgG1 antibody on proA Chip can bind Her2 (Cat.No.10004-H08H) with an affinity constant of 3.270 nM as determined in a SPR assay (Biacore T200) (Routinely tested).4.Captured Trastuzumab (68046-H002) on proA Chip can bind Her2 (Cat.No.10004-H08H) with an affinity constant of 3.727 nM as determined in a SPR assay (Biacore T200)(Routinely tested).5.Loaded Anti-Erbb2 Antibody (Pertuzumab) on ProA Biosensor, can bind Recombinant Human Her2/ERBB2 Protein, His Tag (Cat. No. 10004-H08H) with an affinity constant of 0.382 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).6.Loaded Anti-Erbb2 Antibody (Trastuzumab) on ProA Biosensor, can bind Recombinant Human Her2/ERBB2 Protein, His Tag (Cat. No. 10004-H08H) with an affinity constant of 0.547 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_004439.2
NCBI Gene ID :
Synonyms :
Synonyms :
erb-b2 receptor tyrosine kinase 2
Amino Acid Sequence :
Molecular Weight :
The recombinant human ErbB2 comprises 641 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, rh ErbB2 migrates as an approximately 100-110 kDa protein in SDS-PAGE under reducing conditions.
Purity :
> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CD340 Protein, Human; ENV Protein, Human; ENVW Protein, Human; ERVWE1 Protein, Human; HER-2 Protein, Human; HER-2/neu Protein, Human; HER2 Protein, Human; HERV-7q Protein, Human; HERV-W-ENV Protein, Human; HERV7Q Protein, Human; HERVW Protein, Human; HERVWENV Protein, Human; MLN 19 Protein, Human; MLN19 Protein, Human; NEU Protein, Human; NGL Protein, Human; TKR1 Protein, Human Her2/ERBB2 背景信息 Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy
References & Citations :
Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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