Name :
Recombinant Human GPRC5D Protein (His & FLAG Tag), Detergent
Biological Activity :
Background :
Orphan G-protein-coupled receptor family C group 5 member D (GPRC5D) has seven transmembrane segments forming the core of the receptor, as well as an intracellular C-tail and an extracellular N-terminal domain. GPRC5D is highly and selectively expressed on the surface of CD138+ multiple myeloma (MM) cells, whereas in normal tissues, a much lower expression is limited to plasma cells and certain hard keratinizing tissues. This makes GPRC5D a promising marker for monitoring tumor load and a target antigen for MM-specific T cell-engaging therapies such as bispecific antibodies and CAR-T cells.
Biological Activity :
1.Immobilized Human GPRC5D Protein(Cat:24447-H18H-DD) at 5 μg/mL (100 μL/well) on Nickel coated plate can bind Anti-Human GPRC5D Antibody, Human IgG1, the EC50 is 4.0 ng/ml（QC tested).2.Loaded Anti-GPRC5D Antibody on Protein A Biosensor, can bind GPRC5D Protein(Cat:24447-H18H-DD) with an affinity constant of 58.1 nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
Q9NZD1
NCBI Gene ID :
Synonyms :
Synonyms :
Amino Acid Sequence :
Molecular Weight :
The recombinant human GPRC5D consists of 286 amino acids and predicts a molecular mass of 32.83 kDa.
Purity :
≥ 80 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to three months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the human GPRC5D (Q9NZD1) (Met1-Ser268) was expressed, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus.
Buffer Solution :
Supplied as solution in 10mM HEPES, 50mM NaCl, DDM, pH7.5 with glycerol as protectant.DDM is cytotoxicity and not suitable for cell based experiments.Never remove DDM as it is necessary to the activity of the protein. Do not store the protein without glycerol.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
It is supplied and shipped as liquid with dry ice.
Redissolution :
Synonyms :
GPRC5D Protein, Human GPRC5D 背景信息 Orphan G-protein-coupled receptor family C group 5 member D (GPRC5D) has seven transmembrane segments forming the core of the receptor, as well as an intracellular C-tail and an extracellular N-terminal domain. GPRC5D is highly and selectively expressed on the surface of CD138+ multiple myeloma (MM) cells, whereas in normal tissues, a much lower expression is limited to plasma cells and certain hard keratinizing tissues. This makes GPRC5D a promising marker for monitoring tumor load and a target antigen for MM-specific T cell-engaging therapies such as bispecific antibodies and CAR-T cells.
References & Citations :
1. Eric L. Smith, et al. GPRC5D is a target for the immunotherapy of multiple myeloma with rationally designed CAR T cells. Sci. Transl. Med. 2019. 2. Christie, et al. Preclinical activity and determinants of response of the GPRC5DxCD3 bispecific antibody talquetamab in multiple myeloma. Blood Adv 2021.3. Tatsushi Kodama, et al. Anti-GPRC5D/CD3 Bispecific T-Cell–Redirecting Antibody for the Treatment of Multiple Myeloma. Mol Cancer Ther 1 September 2019.
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