Name :
Recombinant Human Glycerol 3 Phosphate Dehydrogenase/GPD1 Protein (His Tag)
Biological Activity :
Background :
GPD1 (Glycerol-3-Phosphate Dehydrogenase 1) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. GPD1 is a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. It also reduces nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. Meanwhile, GPD1 and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Diseases associated with GPD1 include Hypertriglyceridemia, Transient Infantile, and Myopathy, Distal, 1.
Biological Activity :
Measured by its ability to catalyze dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate, The specific activity is > 400 pmols/min/ug.
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
P21695
NCBI Gene ID :
Synonyms :
Synonyms :
glycerol-3-phosphate dehydrogenase 1 (soluble)
Amino Acid Sequence :
Molecular Weight :
The recombinant human GPD1 consists of 364 amino acids and predicts a molecular mass of 39.4 KDa. It migrates as an approximately 33-37 KDa band in SDS-PAGE under reducing conditions.
Purity :
> 95 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the human GPD1(P21695) (Met1-Met349) was expressed with a polyhistidine tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile 50mM Tris, 10% glycerol, pH 8.0.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
GPD-C Protein, Human; GPDH-C Protein, Human; HTGTI Protein, Human Glycerol 3 Phosphate Dehydrogenase/GPD1 背景信息 GPD1 (Glycerol-3-Phosphate Dehydrogenase 1) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. GPD1 is a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. It also reduces nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. Meanwhile, GPD1 and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Diseases associated with GPD1 include Hypertriglyceridemia, Transient Infantile, and Myopathy, Distal, 1.
References & Citations :
Ou X. et al., 2006, J Mol Biol. 357 (3): 858-69. Ou Xianjin. et al., 2011, Journal of Molecular Biology. 357 (3): 858-69. Harding Jr. et al., 1975, Biochem Journal. 146: 223-9.
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