Name :
Recombinant Human GAPDH Protein (His Tag)
Biological Activity :
Background :
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer’s disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson’s disease, binds to GAPDH and displays neuroprotective actions.
Biological Activity :
Measured by its ability to catalyze D-glyceraldehyde 3-phosphate to 3-phosphoglycerate ,The specific activity is >2500pmols/min/ug.
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
P04406
NCBI Gene ID :
Synonyms :
Synonyms :
glyceraldehyde-3-phosphate dehydrogenase
Amino Acid Sequence :
Molecular Weight :
The recombinant human GAPDH comprises 346 amino acids and migrates as an approximately 38 kDa band in SDS-PAGE under reducing conditions as predicted.
Purity :
> 93 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the human GAPDH (P04406) (Met 1-Glu 335) was expressed, with a polyhistidine tag at the N-terminus.
Buffer Solution :
Supplied as sterile 50mM Tris, 30% glycerol, pH 7.5Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Liquid. It is shipped out with blue ice.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
G3PD Protein, Human; GAPD Protein, Human; HEL-S-162eP Protein, Human GAPDH 背景信息 Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer’s disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson’s disease, binds to GAPDH and displays neuroprotective actions.
References & Citations :
Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) – Molecular Basis of Disease. 1762 (5): 502-9.Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
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