Name :
Recombinant Human GALE Protein (His Tag)

Biological Activity :

Background :
UDP galactose-4′-epimerase, also known as GALE, enables the body to process a simple sugar called galactose, which is present in small amounts in many foods. Galactose is primarily part of a larger sugar called lactose, which is found in all dairy products and many baby formulas. UDP galactose-4′-epimerase catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. Defects in GALE causes epimerase-deficiency galactosemia (EDG), also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation.

Biological Activity :
Testing in progress

Expression Host :
Human

Source :
E. coli

Tag :

Protein Accession No. :
Q14376

NCBI Gene ID :

Synonyms :

Synonyms :
UDP-galactose-4-epimerase

Amino Acid Sequence :

Molecular Weight :
The recombinant human GALE consists of 363 amino acids and predicts a molecular mass of 40.1 KDa. It migrates as an approximately 36 KDa band in SDS-PAGE under reducing conditions.

Purity :
> 95 % as determined by SDS-PAGE

State of Matter :

Product Concentration :

Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Endotoxin Level :
Please contact us for more information.

Protein Construction :
A DNA sequence encoding the mature form of human GALE (Q14376) (Met1-Ala348) was expressed with a polyhistidine tag at the N-terminus.

Buffer Solution :
Lyophilized from sterile 20mM HEPES, 150mM NaCl, 10% Glycerol, pH 7.5.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.

Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Synonyms :
SDR1E1 Protein, Human GALE 背景信息 UDP galactose-4′-epimerase, also known as GALE, enables the body to process a simple sugar called galactose, which is present in small amounts in many foods. Galactose is primarily part of a larger sugar called lactose, which is found in all dairy products and many baby formulas. UDP galactose-4′-epimerase catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. Defects in GALE causes epimerase-deficiency galactosemia (EDG), also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation.

References & Citations :
Kim W. et al., 2011, Mol Cell. 44 (2): 325-40. Lee KA. et al., 2011,. J Biol Chem. 286 (48): 41530-8. McCorvie TJ. et al., 2012, Biochim Biophys Acta. 1822 (10): 1516-26.

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