Name :
Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag), Amine-Labeled Biotinylated
Biological Activity :
Background :
Biological Activity :
1.Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag), Biotinylated (Cat. CT009-H08H-B) bound to IgG1 Antibody was 4.6 nM.2.Loaded Recombinant Human FCGRT & B2M Heterodimer Protein, His Tag, Amine-Labeled Biotinylated (Cat. No. CT009-H08H-B) on SA Biosensor, can bind Anti-Erbb2 Antibody, human IgG1 with an affinity constant of 11.2 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
P55899 & P61769
NCBI Gene ID :
Synonyms :
Synonyms :
Fc fragment of IgG, receptor, transporter, alpha
Amino Acid Sequence :
Molecular Weight :
The recombinant heterodimer of human FCGRT/B2M consists of 385 (285 + 100) amino acids and predicts a molecular mass of 43.5 (31.8 + 11.7) kDa.
Purity :
> 95 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (P55899) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified. The purified protein was biotinylated in vitro.
Buffer Solution :
Lyophilized from sterile PBS.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
alpha-chain Protein, Human; FCRN Protein, Human FCGRT & B2M Heterodimer 背景信息
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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