Name :
Recombinant Human Fas Ligand/TNFSF6 Protein (His Tag)
Biological Activity :
Background :
Fas Ligand, also known as FASLG and CD95L, is the ligand for FAS. It is a transmembrane protein which binds to TNFRSF6/FAS. Interaction of FAS with fas Ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Fas Ligand may be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.
Biological Activity :
Immobilized Human Fas/CD95 hFc (Cat:10217-H02H) at 2 μg/mL (100 μL/well) can bind Human Fas Ligand His (Cat:10244-H07Y), the EC50 of Human Fas Ligand His (Cat:10244-H07Y) is 40-200 ng/mL.
Expression Host :
Human
Source :
Yeast
Tag :
Protein Accession No. :
NP_000630.1
NCBI Gene ID :
Synonyms :
Synonyms :
Fas ligand
Amino Acid Sequence :
Molecular Weight :
The recombinant human human FASLG consists of 168 amino acids and predicts a molecular mass of 19.3 KDa. It migrates as an approximately 26 KDa band in SDS-PAGE under reducing conditions.
Purity :
> 90 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding human FASLG (NP_000630.1) (Pro134-Leu281) was expressed with a polyhistidine tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
ALPS1B Protein, Human; APT1LG1 Protein, Human; APTL Protein, Human; CD178 Protein, Human; CD95-L Protein, Human; CD95L Protein, Human; FASL Protein, Human; FASLG Protein, Human; TNFSF6 Protein, Human; TNLG1A Protein, Human Fas Ligand/TNFSF6 背景信息 Fas Ligand, also known as FASLG and CD95L, is the ligand for FAS. It is a transmembrane protein which binds to TNFRSF6/FAS. Interaction of FAS with fas Ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Fas Ligand may be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.
References & Citations :
Pitti R M. et al., 1998, Nature. 396 (6712): 699-703. Hane M. et al., 1995, FEBS Lett. 373 (3): 265-8. Schneider P. et al., 1997, J Biol Chem. 272 (30): 18827-33.
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