Name :
Recombinant Human EGF-L6 Protein (His & FLAG Tag)
Biological Activity :
Background :
EGFL6, also known as EGF-L6, belongs to the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. EGFL6 contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. EGFL6 gene is expressed early during development, and its expression has been detected in lung and meningioma tumors.
Biological Activity :
Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 × 10E4 cells/well areadded to CD4-coated plates (1.25μg/mL and 100μL/well),approximately 40%-60% will adhere specifically after 30 minutes at 37℃.
Expression Host :
Human
Source :
Baculovirus-insect cells
Tag :
Protein Accession No. :
NP_056322.2
NCBI Gene ID :
Synonyms :
Synonyms :
EGF-like-domain, multiple 6
Amino Acid Sequence :
Molecular Weight :
The recombinant human EGFL6 consists of 551 amino acids and has a calculated molecular mass of 61.5 kDa.
Purity :
> 65 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the human EGFL6 (NP_056322.2) (Asn22-Asp553) was expressed with the flag tag at the N-terminus and the histidine tag at the C-terminus.
Buffer Solution :
Supplied as sterile 20 mM Tris, 500 mM NaCl, 20 % glycerol, pH 7.4.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Liquid. It is shipped out with blue ice.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
MAEG Protein, Human; W80 Protein, Human EGF-L6 背景信息 EGFL6, also known as EGF-L6, belongs to the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. EGFL6 contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. EGFL6 gene is expressed early during development, and its expression has been detected in lung and meningioma tumors.
References & Citations :
Kimura K. et al., 2006, Genome Res. 16 (1): 55-65. Lee SH. et al., 2008, Pathology. 40 (1): 95-7. Oberauer R. et al., 2010, Mol Cell Biochem. 343 (1-2): 257-69.
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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