Name :
Recombinant Human CHST11 Protein (His Tag)
Biological Activity :
Background :
CHST11, also known as C4ST-1, belongs to the sulfotransferase 2 family. CHST11 localizes to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. A chromosomal translocation involving CHST11 gene and IgH, t(12;14)(q23;q32), has been reported in a patient with B-cell chronic lymphocytic leukemia.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
Q9NPF2-2
NCBI Gene ID :
Synonyms :
Synonyms :
carbohydrate (chondroitin 4) sulfotransferase 11
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant human CHST11 consists of 323 amino acids and predicts a molecular mass of 38.4 KDa. The apparent molecular mass of the protein is approximately 43 KDa in SDS-PAGE under reducing conditions.
Purity :
> 95 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human CHST11 (Q9NPF2-2) (Met36-Glu347) was expressed with a C-terminal polyhistidine tag.
Buffer Solution :
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
C4ST Protein, Human; C4ST-1 Protein, Human; C4ST1 Protein, Human; HSA269537 Protein, Human CHST11 背景信息 CHST11, also known as C4ST-1, belongs to the sulfotransferase 2 family. CHST11 localizes to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. A chromosomal translocation involving CHST11 gene and IgH, t(12;14)(q23;q32), has been reported in a patient with B-cell chronic lymphocytic leukemia.
References & Citations :
Hiraoka N. et al., 2000, J Biol Chem. 275 (26): 20188-96.Schmidt HH. et al., 2004, Oncogene. 23 (41): 6991-6.Okuda T. et al., 2001, J Biochem. 128 (5): 763-70.
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