Name :
Recombinant Human 5′-nucleotidase/CD73/NT5E Protein (His Tag), HPLC-verified
Biological Activity :
Background :
5′-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5′-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
Biological Activity :
1. Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) (Cat. 10904-H08H) bound MEDI9447 was 0.3 nM.2. Measured by its ability to hydrolyze the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP). The specific activity is > 15,000 pmoles/min/μg.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_002517.1
NCBI Gene ID :
Synonyms :
Synonyms :
5′-nucleotidase, ecto (CD73)
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant human NT5E consists of 532 amino acids with the predicted molecular mass of 59.2 kDa. It migrates as an approximately 63 kDa protein due to glycosylation by SDS-PAGE under reducing conditions. The molecular weight of this protein is around 125.4 kDa kDa verified by SEC-MALS (Routinely tested).
Purity :
≥ 85 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 90% as determined by SEC-MALS(Routinely tested).
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human NT5E (NP_002517.1) (Met1-Lys547) was expressed fused with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2，pH 7.5，with Glycerol as protectants.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CALJA Protein, Human; CD73 Protein, Human; E5NT Protein, Human; eN Protein, Human; eNT Protein, Human; NT Protein, Human; NT5 Protein, Human; NTE Protein, Human 5′-nucleotidase/CD73/NT5E 背景信息 5′-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5′-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
References & Citations :
Resta R. et al., 1997, Cell Signal. 9 (2): 131-9. Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90. Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55. Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.
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