Name :
Recombinant Human CD64/FCGR1 Protein (ECD, His Tag), HPLC-verified
Biological Activity :
Background :
High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.
Biological Activity :
1.Immobilized Anti-CD20(Ro) Antibody (Rituximab) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD64 / FCGR1A Protein (His Tag)(Cat:10256-H08H), the EC50 0.6-2.0 ng/mL.2.Captured Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 5.5 nM as determined in an SPR assay (Biacore T200).3.Captured Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 13.6 nM as determined in an SPR assay (Biacore T200).4.Loaded Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 8.5 nM as determined in a BLI assay (Sartorius Octet Red384) .5.Loaded Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 4.7 nM as determined in a BLI assay (Sartorius Octet Red384).6.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) with an affinity constant of 0.38 nM as determined in a BLI assay (Sartorius Octet Red384).7.The purity of Human CD64 / FCGR1A Protein (His Tag) (Cat.10256-H08H) is more than 95% and the molecular weight of this protein is around 38-52 kDa verified by SEC-MALS.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_000557.1
NCBI Gene ID :
Synonyms :
Synonyms :
Fc receptor, IgG, high affinity I
Amino Acid Sequence :
Molecular Weight :
The recombinant human CD64A consists of 284 amino acids and has a predicted molecular mass of 32 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant human CD64A is approximately 45-60 kDa due to glycosylation.
Purity :
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the extracellular domain of human CD64A (NP_000557.1) (Met 1-Pro 288) was expressed, fused with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CD64 Protein, Human; Fc gamma RI Protein, Human CD64/FCGR1 背景信息 High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.
References & Citations :
Allen J.M., et al.,(1988), Nucleotide sequence of three cDNAs for the human high affinity Fc receptor (FcRI). Nucleic Acids Res. 16:11824-11824.Allen J.M., et al., (1989), Isolation and expression of functional high-affinity Fc receptor complementary DNAs.Science 243:378-381.Porges A.J., et al.,(1992), Novel Fc gamma receptor I family gene products in human mononuclear cells.J. Clin. Invest. 90:2102-2109.
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