Product Name: Anti-CDC25B (AB2) antibody produced in rabbit
Synonym: Anti-Cell division cycle 25 homolog B (S. pombe)
Product Type: Chemical
CAS NO: 1033040-23-1IRE1 inhibitors
antibody Form: affinity isolated antibody
application(s)
immunohistochemistry: suitable
western blot: suitable
biological source
rabbit
clone
polyclonal
Concentration: 0.5 mg – 1 mg/mL
Conjugate: unconjugated
Form: buffered aqueous solution
Mol wt: mol wt 65 kDa
NCBI accession no.
NP_004349
Shipped in: wet ice
species reactivity
guinea pig, horse, rabbit, canine, pig
Storage temp.: −20°C
Application: Anti- CDC25B (AB2) antibody produced in rabbit is suitable for western blotting at a concentration of 1 μg/ml. For immunohistochemistry of paraffin-embedded tissue sections, a concentration of 4-8 μg/ml is suitable.
Biochem/physiol Actions:
CDC25 phosphatases regulate the progression of cell cycle. The activity of CDC25B is important for G2/M transition, cell migration, invasion and survival. The PKA/CDC25B pathway plays a crucial role in meiotic arrest of mouse oocytes. The activity of CDC25B is regulated by 14-3-3 proteins.
Immunogen:
The immunogen for anti-CDC25B antibody: synthetic peptide derected towards the N terminal of human CDC25B
Physical Form: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence:
Synthetic peptide located within the following region: MEVPQPEPAPGSALSPAGVCGGAQR
RIDADR: NONH for all modes of transport
WGK Germany: 3
Storage Temp.: −20°C
UNSPSC
12352203
PubMed ID:http://jpet.aspetjournals.org/content/327/3/910