Name :
Recombinant Human SERPINB4 Protein (His Tag)
Biological Activity :
Background :
Overexpression of SERPINB4 in tumor cells inhibited recombinant GrM-induced as well as NK cell-mediated cell death and this inhibition depended on the reactive center loop of the serpin.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
P48594
NCBI Gene ID :
Synonyms :
Synonyms :
serpin peptidase inhibitor, clade B (ovalbumin), member 4
Amino Acid Sequence :
Molecular Weight :
The recombinant human SERPINB4 consists of 409 amino acids and predicts a molecular mass of 47.1 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 88 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human SERPINB4 (P48594) (Met 1-Pro 390) was expressed, with a polyhistidine tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 20% gly, 1mM EDTA, 1mM DTTPlease contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
LEUPIN Protein, Human; PI11 Protein, Human; SCCA-2 Protein, Human; SCCA1 Protein, Human; SCCA2 Protein, Human; Serpin B4 Protein, Human SERPINB4 背景信息 Overexpression of SERPINB4 in tumor cells inhibited recombinant GrM-induced as well as NK cell-mediated cell death and this inhibition depended on the reactive center loop of the serpin.
References & Citations :
de Koning PJ, et al. (2011) Intracellular Serine Protease Inhibitor SERPINB4 Inhibits Granzyme M-Induced Cell Death. PLoS One. 6(8): e22645.Chechlinska M, et al. (2010) Squamous cell carcinoma antigen 1 and 2 expression in cultured normal peripheral blood mononuclear cells and in vulvar squamous cell carcinoma. Tumour Biol. 31(6): 559-67.Shiiba M, et al. (2010) Down-regulated expression of SERPIN genes located on chromosome 18q21 in oral squamous cell carcinomas. Oncol Rep. 24(1): 241-9. en, Der p 1. J Biol Chem. 279(7): 5081-7.Murakami A, et al. (2008) Relationship between decreased expression of squamous cell carcinoma antigen 2 and E-cadherin in primary cervical cancer lesions and lymph node metastasis. Oncol Rep. 19(1): 99-104.Silverman GA, et al. (1998) SCCA1 and SCCA2 are proteinase inhibitors that map to the serpin cluster at 18q21.3. Tumour Biol. 19(6): 480-7.
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