Name :
Recombinant Human SEMA6A Protein (His Tag)
Biological Activity :
Background :
SEMA6A, the first identified class 6 semaphorin, is contributed to regulate vascular development and adult angiogenesis. SEMA6A could inhibit proliferation, migration, and invasion in different glioma cell lines. That SEMA6A may be a potential prognostic biomarker in the treatment of GBM. Among different semaphorins tested by reverse transcriptase-polymerase chain reaction in human immune cells, the expression of class 6 transmembrane semaphorin SEMA6A was restricted to dendritic cells (DCs).
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_065847.1
NCBI Gene ID :
Synonyms :
Synonyms :
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
Amino Acid Sequence :
Molecular Weight :
The recombinant human SEMA6A consists of 642 amino acids and predictes a molecular mass of 72.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SEMA6A is approximately 85-95 kDa due to glycosylation.
Purity :
> 90 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human SEMA6A (NP_065847.1) extracellular domain (Met 1-Thr 649) was expressed, with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
HT018 Protein, Human; SEMA Protein, Human; SEMA6A1 Protein, Human; SEMAQ Protein, Human; VIA Protein, Human SEMA6A 背景信息 SEMA6A, the first identified class 6 semaphorin, is contributed to regulate vascular development and adult angiogenesis. SEMA6A could inhibit proliferation, migration, and invasion in different glioma cell lines. That SEMA6A may be a potential prognostic biomarker in the treatment of GBM. Among different semaphorins tested by reverse transcriptase-polymerase chain reaction in human immune cells, the expression of class 6 transmembrane semaphorin SEMA6A was restricted to dendritic cells (DCs).
References & Citations :
Klostermann A., et al., 2000, J. Biol. Chem. 275:39647-53. Nagase T., et al., 2000, DNA Res. 7:65-73. Schmutz J., et al., 2004, Nature 431:268-74. Neufeld, G. et al., 2005, Front Biosci. 10 : 751-60. Fiore,R. et al., 2005, Mol Cell Biol. 25 (6):2310-9.
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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