Name :
Recombinant Human S100A1 Protein (hFc Tag)
Biological Activity :
Background :
S100A1 is a Ca2+ binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. This protein has been shown to function in the processes including stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.
Biological Activity :
Measured by its ability to bind biotinylated Human Fc-S100B (cat: 10181-H01H) in functional Elisa.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_006262.1
NCBI Gene ID :
Synonyms :
Synonyms :
S100 calcium binding protein A1
Amino Acid Sequence :
Molecular Weight :
The recombinant human Fc/S100A1 is a disulfide-linked homodimeric protein. The reduced monomer consists of 330 amino acids and has a predicted molecular mass of 37.1 kDa. As a result of glycosylation, the apparent molecular mass of rhFc/S100A1 monomer is approximately 40 kDa in SDS-PAGE under reducing conditions.
Purity :
> 95 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human S100A1 (NP_006262.1) (Gly 2-Ser94) was expressed with the N-terminal fused Fc region of human IgG1.
Buffer Solution :
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
S100 Protein, Human; S100-alpha Protein, Human; S100A Protein, Human S100A1 背景信息 S100A1 is a Ca2+ binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. This protein has been shown to function in the processes including stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.
References & Citations :
Remppis, A .et al., 1996, Biochim. Biophs. Acta. 1313: 253-257. Most, P. et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98: 13889-13894 Okada, M. et al., 2004, J. Biol. Chem. 279: 4221-4233. Schafer, W.E. et al., 1995, Genomics. 25: 638-643. Landar, A. et al., 1996, Cell. Calcium. 20: 279-285.
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