Name :
Recombinant Human PU.1/SPI1 Protein (His Tag)
Biological Activity :
Background :
SPI1 (also known as PU.1) is a dominant but transient regulator in early T-cell precursors and a potent transcriptional controller of developmentally important pro-T-cell genes. SPI1 is an essential transcription factor (TF) for the hematopoietic lineage, in which its expression is tightly controlled through a -17-kb upstream regulatory region and a promoter region. The E26 transformation-specific (ETS) family transcription factor PU.1/Spi1 acts as a master regulator of myeloid and lymphoid development. PU.1-deficient mice show a complete loss of microglia, indicating that PU.1 plays a pivotal role in micro-gliogenesis. The Spi1/PU.1 transcription factor is a key regulator of many steps of hematopoiesis, and limits self-renewal of hematopoietic stem cells. The deregulation of its expression or activity contributes to leukemia, in which Spi1 can be either an oncogene or a tumor suppressor.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
P17947
NCBI Gene ID :
Synonyms :
Synonyms :
spleen focus forming virus (SFFV) proviral integration oncogene
Amino Acid Sequence :
Molecular Weight :
The recombinant Human SPI1 consists of 277 amino acids and predicts a molecular mass of 32.04 kDa.
Purity :
≥ 90 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the Human SPI1 (P17947) (Met1-His270) was expressed, with a polyhistidine tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile PBS, 0.05% SKL.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
OF Protein, Human; PU.1 Protein, Human; SFPI1 Protein, Human; SPI-1 Protein, Human; SPI-A Protein, Human PU.1/SPI1 背景信息 SPI1 (also known as PU.1) is a dominant but transient regulator in early T-cell precursors and a potent transcriptional controller of developmentally important pro-T-cell genes. SPI1 is an essential transcription factor (TF) for the hematopoietic lineage, in which its expression is tightly controlled through a -17-kb upstream regulatory region and a promoter region. The E26 transformation-specific (ETS) family transcription factor PU.1/Spi1 acts as a master regulator of myeloid and lymphoid development. PU.1-deficient mice show a complete loss of microglia, indicating that PU.1 plays a pivotal role in micro-gliogenesis. The Spi1/PU.1 transcription factor is a key regulator of many steps of hematopoiesis, and limits self-renewal of hematopoietic stem cells. The deregulation of its expression or activity contributes to leukemia, in which Spi1 can be either an oncogene or a tumor suppressor.
References & Citations :
Ungerbäck J, et al. (2018) Pioneering, chromatin remodeling, and epigenetic constraint in early t-cell gene regulation by spi1 (pu.1). Genome Res 28 (10): 1508-1519.Satoh J, et al. (2014) A comprehensive profile of chip-seq-based pu.1/spi1 target genes in microglia. Gene Regul Syst Bio 8 127-139.Goyal S, et al. (2017) Runx1 induces DNA replication independent active DNA demethylation at spi1 regulatory regions. BMC Mol Biol 18 (1): 9.Delestré L, et al. (2017) Senescence is a spi1-induced anti-proliferative mechanism in primary hematopoietic cells. Haematologica 102 (11): 1850-1860.
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