Name :
Recombinant Human PSMA/FOLH1 Protein (His Tag), Amine-Labeled Biotinylated, HPLC-verified
Biological Activity :
Background :
Glutamate carboxypeptidase 2, also known as Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, Prostate-specific membrane antigen, GCPII, PSMA, FOLH1, and NAALAD1, is a single-pass type II membrane protein which belongs to thepeptidase M28 family and M28B subfamily. FOLH1 is highly expressed in prostate epithelium. It is detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon, brain (at protein level), and the capillary endothelium of a variety of tumors. FOLH1 has both folate hydrolase and N-acetylated alpha linked acidic dipeptidase (NAALADase) activity. It has a preference for tri-alpha-glutamate peptides. Genetic variation in FOLH1 may be associated with low folate levels and consequent hyperhomocysteinemia. This condition can result in increased risk of cardiovascular disease, neural tube defects, and cognitive deficits. FOLH1 also shows a promising role in directed imaging and therapy of recurrent or metastatic disease. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy
Biological Activity :
Immobilized anti-PSMA at 2 μg/mL (100 μL/well) can bind Recombinant Human PSMA / FOLH1 Protein (His Tag), Biotinylated (Cat: 15877-H07H-B), the EC50 is 1.2-3.6 ng/mL.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_004467.1
NCBI Gene ID :
Synonyms :
Synonyms :
folate hydrolase (prostate-specific membrane antigen) 1
Amino Acid Sequence :
Molecular Weight :
The recombinant human FOLH1 consists of 726 amino acids and predicts a molecular mass of 81.7 kDa.
Purity :
≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the human FOLH1 (NP_004467.1) (Lys44-Ala750) was expressed with a polyhistidine tag at the N-terminus. The purified protein was biotinylated in vitro.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
FGCP Protein, Human; FOLH Protein, Human; GCP2 Protein, Human; GCPII Protein, Human; mGCP Protein, Human; NAALAD1 Protein, Human; NAALAdase Protein, Human; PSM Protein, Human; PSMA Protein, Human PSMA/FOLH1 背景信息 Glutamate carboxypeptidase 2, also known as Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, Prostate-specific membrane antigen, GCPII, PSMA, FOLH1, and NAALAD1, is a single-pass type II membrane protein which belongs to thepeptidase M28 family and M28B subfamily. FOLH1 is highly expressed in prostate epithelium. It is detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon, brain (at protein level), and the capillary endothelium of a variety of tumors. FOLH1 has both folate hydrolase and N-acetylated alpha linked acidic dipeptidase (NAALADase) activity. It has a preference for tri-alpha-glutamate peptides. Genetic variation in FOLH1 may be associated with low folate levels and consequent hyperhomocysteinemia. This condition can result in increased risk of cardiovascular disease, neural tube defects, and cognitive deficits. FOLH1 also shows a promising role in directed imaging and therapy of recurrent or metastatic disease. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy
References & Citations :
Israeli R.S., et al.,(1993), Molecular cloning of a complementary DNA encoding a prostate-specific membrane antigen. Cancer Res. 53:227-230. Su S.L., et al., (1995), Alternatively spliced variants of prostate-specific membrane antigen RNA: ratio of expression as a potential measurement of progression.Cancer Res. 55:1441-1443. O’Keefe D.S., et al.,(1998), Mapping, genomic organization and promoter analysis of the human prostate-specific membrane antigen gene.Biochim. Biophys. Acta 1443:113-127.
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