Name :
Recombinant Human Podoplanin Protein (ECD, His & hFc Tag)
Biological Activity :
Background :
Podoplanin, also known as PDPN, is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined. Alternatively spliced transcript variants encoding different isoforms have been identified.PDPN is a mucin-type glycoprotein negatively charged by extensive O-glycosylation and a high content of sialic acid, which expresses the adhesive property. It is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. PDPN may contribute to form odontoblastic fiber or function as the anchorage to the tooth development and in proliferating epithelial cells of cervical loop and apical bud. The intensity of podoplanin expression is negatively correlated with the expression of CD34 and factor VIII. Podoplanin would be useful as a diagnostic marker for epithelioid hemangioendothelioma in liver tumors. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy
Biological Activity :
1. Measured by its binding ability in a functional ELISA. 2. Immobilized human Podoplanin (Cat: 11065-H03H) at 10 μg/mL (100 μl/well) can bind biotinylated human CLEC1B-His (Cat:10976-H07H), The EC50 of biotinylated human CLEC1B-His is 0.4-1.0 μg/mL.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
Q86YL7-1
NCBI Gene ID :
Synonyms :
Synonyms :
podoplanin
Amino Acid Sequence :
Molecular Weight :
The recombinant human PDPN/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 349 amino acids with a predicted molecular mass of 38.4 kDa. As a result of glycosylation, rh PDPN/Fc monomer migrates as an approximately 50.2 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 95 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the extracellular domain of human PDPN (Q86YL7-1) (Met1-Lys123) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
AGGRUS Protein, Human; GP36 Protein, Human; Gp38 Protein, Human; GP40 Protein, Human; HT1A-1 Protein, Human; OTS8 Protein, Human; PA2.26 Protein, Human; T1A Protein, Human; T1A-2 Protein, Human; T1A2 Protein, Human; TI1A Protein, Human Podoplanin 背景信息 Podoplanin, also known as PDPN, is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined. Alternatively spliced transcript variants encoding different isoforms have been identified.PDPN is a mucin-type glycoprotein negatively charged by extensive O-glycosylation and a high content of sialic acid, which expresses the adhesive property. It is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. PDPN may contribute to form odontoblastic fiber or function as the anchorage to the tooth development and in proliferating epithelial cells of cervical loop and apical bud. The intensity of podoplanin expression is negatively correlated with the expression of CD34 and factor VIII. Podoplanin would be useful as a diagnostic marker for epithelioid hemangioendothelioma in liver tumors. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy
References & Citations :
Kimura, N. et al., 2005, Pathol Int. 55 (2): 83-86. Ordóñez, N.G., 2006, Adv Anat Pathol. 13 (2): 83-88. Wicki, A. et al., 2007, Br. J. Cancer. 96 (1): 1-5. Fujii,T. et al., 2008, Mod Pathol. 21 (2): 125-130. Sawa,Y. et al., 2008, Acta Histochem Cytochem. 41 (5):121-126. Kaddu, S. et al., 2009, Am J Dermatopathol. 31 (2): 137-139.
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
DNAM-1 Proteinsupplier
IGFBP-1 ProteinFormulation
Popular categories:
Angiopoietin Like 4
DNGR-1/CD370