Name :
Recombinant Human Nucleoside phosphorylase/PNP Protein (His Tag)
Biological Activity :
Background :
Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells. Purine nucleoside phosphorylase is a transferase that catalyzes the addition of phosphate and removal of a purine base from guanosine and similar nucleosides.PNP defects result in metabolic abnormalities and fatal T cell immunodeficiency. Purine nucleoside phosphorylase (PNP) is a target for leukemia, gout, and autoimmune disorders.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
P00491
NCBI Gene ID :
Synonyms :
Synonyms :
purine nucleoside phosphorylase
Amino Acid Sequence :
Molecular Weight :
The recombinant human PNP consisting of 299 amino acids and has a calculated molecular mass of 33.5 kDa as estimated in SDS-PAGE under reducing conditions.
Purity :
> 97 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the human PNP (P00491) (Met 1-Ser 289) was fused with a polyhistide tag at the C-terminus.
Buffer Solution :
Supplied as sterile PBS, 25% glycerol, pH 7.5Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Liquid. It is shipped out with blue ice.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
NP Protein, Human; PRO1837 Protein, Human; PUNP Protein, Human Nucleoside phosphorylase/PNP 背景信息 Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells. Purine nucleoside phosphorylase is a transferase that catalyzes the addition of phosphate and removal of a purine base from guanosine and similar nucleosides.PNP defects result in metabolic abnormalities and fatal T cell immunodeficiency. Purine nucleoside phosphorylase (PNP) is a target for leukemia, gout, and autoimmune disorders.
References & Citations :
de Azevedo,W.F. et al., 2003, Biochem Biophys Res Commun. 312 (3): 767-72. Canduri,F. et al., 2005, Acta Crystallogr D Biol Crystallogr. 61 (Pt 7): 856-62. Perera,G.K. et al., 2005,Clin Exp Dermatol. 30 (1):27-9.
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