Name :
SARS-CoV-2 (2019-nCoV) NSP10-His Recombinant Protein
Biological Activity :
Background :
NSP10 is a major regulator of coronavirus replicase function. NSP10 contains two zinc fingers and binds and stimulates both NSP14 and NSP16 activities. Researchers has found that the nsp10 surface that interacts with nsp14 and nsp16 and possibly other subunits of the viral replication complex may be a target for the development of antiviral compounds against pathogenic coronaviruses.
Biological Activity :
Testing in progress
Expression Host :
SARS-CoV-2
Source :
E. coli
Tag :
Protein Accession No. :
YP_009725306.1
NCBI Gene ID :
Synonyms :
Synonyms :
Amino Acid Sequence :
Molecular Weight :
The recombinant SARS-CoV-2 (2019-nCoV) NSP10 Protein (His tag) consists of 146 amino acids and predicts a molecular mass of 15.7 kDa.
Purity :
> 95 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) NSP10 (YP_009725306.1) (Ala1-Gln139) was expressed with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
Mickaël Bouvet,et al.Coronavirus Nsp10, a Critical Co-factor for Activation of Multiple Replicative Enzymes.THE JOURNAL OF BIOLOGICAL CHEMISTRY.2014Yuanyuan Ma,et al.Structural basis and functional analysis of the SARS coronavirus nsp14-nsp10 complex. Proc Natl Acad Sci USA. 2015Etienne Decroly,et al.Crystal structure and functional analysis of the SARS-coronavirus RNA cap 2′-O-methyltransferase nsp10/nsp16 complex. PLoS Pathog.2011
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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