Name :
Recombinant Human PGA4 Protein (hFc Tag)
Biological Activity :
Background :
PGA4 (Pepsinogen 4, group I), or Pepsinogen A, is a member of the peptidase A1 family. Pepsin is expressed as a pro-form zymogen, pepsinogen, whose primary structure has an additional 44 amino acids. Pepsin is stored as pepsinogen so it will only be released when needed, and does not digest the body’s own proteins in the stomach’s lining. Five types of zymogens of pepsins, gastric digestive proteinases, are known: pepsinogens A, B, and F, progastricsin, and prochymosin. There are two major groups of pepsinogen, namely pepsinogen A (PGA) and pepsinogen C (PGC) (or progastricsin), and each frequently has isozymogens. The PGA3, PGA4 and PGA5 genes encode identical human pepsinogen A enzymes.
Biological Activity :
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2, AnaSpec, Catalog # 27114.The specific activity is > 3000 pmoles/min/μg.(Activation description: The enzyme achieves its activity under acidic pH)
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_001073276.1
NCBI Gene ID :
Synonyms :
Synonyms :
pepsinogen 4, group I (pepsinogen A)
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant human PGA4/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 614 amino acids and has a predicted molecular mass of 67.3 kDa. The apparent molecular mass of rhPGA/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.
Purity :
> 80 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human PGA4 (NP_001073276.1) (Met 1-Ala 388) was fused with the Fc region of human IgG1 at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
FLJ58952 Protein, Human; FLJ77962 Protein, Human; PGA3 Protein, Human; PGA4 Protein, Human; PGA5 Protein, Human PGA4 背景信息 PGA4 (Pepsinogen 4, group I), or Pepsinogen A, is a member of the peptidase A1 family. Pepsin is expressed as a pro-form zymogen, pepsinogen, whose primary structure has an additional 44 amino acids. Pepsin is stored as pepsinogen so it will only be released when needed, and does not digest the body’s own proteins in the stomach’s lining. Five types of zymogens of pepsins, gastric digestive proteinases, are known: pepsinogens A, B, and F, progastricsin, and prochymosin. There are two major groups of pepsinogen, namely pepsinogen A (PGA) and pepsinogen C (PGC) (or progastricsin), and each frequently has isozymogens. The PGA3, PGA4 and PGA5 genes encode identical human pepsinogen A enzymes.
References & Citations :
Kageyama T. (2002) Pepsinogens, progastricsins, and prochymosins: structure, function, evolution, and development. Cell Mol Life Sci. 59(2): 288-306.Takahashi K. (1992) Gene structures of pepsinogens A and C. Scand J Clin Lab Invest Suppl. 210: 97-110.
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