V5-tag monoclonal antibody (E10/V4RR) (HRP conjugate)

Description:
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the epitope tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered protein, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4. Figure 1. Western Blot with V5-tag, mAb (E10/V4RR) (HRP conjugate ) (Prod.2244602-23-9 Technical Information no ENZ-ABS287)Western blot analysis of V5 Epitope Tag was performed by loading various amounts of E.1774353-12-6 custom synthesis coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel.PMID:26574782 Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with V5-tag, mAb (E10/V4RR) (HRP conjugate ) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed. Figure 1. Western Blot with V5-tag, mAb (E10/V4RR) (HRP conjugate ) (Prod. no ENZ-ABS287)Western blot analysis of V5 Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with V5-tag, mAb (E10/V4RR) (HRP conjugate ) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed.