Product Name :
Pitstop 2
Description:
Pitstop 2 is a clathrin inhibitor which inhibits clathrin-mediated endocytosis (CME) by associating with the terminal domain of clathrin. Pitstop 2 has the potential for anti-cancer research.
CAS:
1419320-73-2
Molecular Weight:
473.36
Formula:
C20H13BrN2O3S2
Chemical Name:
(Z)-N-(5-(4-bromobenzylidene)-4-oxo-4, 5-dihydrothiazol-2-yl)naphthalene-1-sulfonamide
Smiles :
O=C1N=C(NS(=O)(=O)C2=CC=CC3=CC=CC=C32)S/C/1=C\C1C=CC(Br)=CC=1
InChiKey:
CGDLWHGPJPVPDU-ATVHPVEESA-N
InChi :
InChI=1S/C20H13BrN2O3S2/c21-15-10-8-13(9-11-15)12-17-19(24)22-20(27-17)23-28(25,26)18-7-3-5-14-4-1-2-6-16(14)18/h1-12H,(H,22,23,24)/b17-12-
Purity:
≥98% (or refer to the Certificate of Analysis)
Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition :
Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life:
≥360 days if stored properly.
Stock Solution Storage:
0 – 4 oC for 1 month or refer to the Certificate of Analysis.
Additional information:
Pitstop 2 is a clathrin inhibitor which inhibits clathrin-mediated endocytosis (CME) by associating with the terminal domain of clathrin. Pitstop 2 has the potential for anti-cancer research.|Product information|CAS Number: 1419320-73-2|Molecular Weight: 473.36|Formula: C20H13BrN2O3S2|Chemical Name: (Z)-N-(5-(4-bromobenzylidene)-4-oxo-4, 5-dihydrothiazol-2-yl)naphthalene-1-sulfonamide|Smiles: O=C1N=C(NS(=O)(=O)C2=CC=CC3=CC=CC=C32)S/C/1=C\C1C=CC(Br)=CC=1|InChiKey: CGDLWHGPJPVPDU-ATVHPVEESA-N|InChi: InChI=1S/C20H13BrN2O3S2/c21-15-10-8-13(9-11-15)12-17-19(24)22-20(27-17)23-28(25,26)18-7-3-5-14-4-1-2-6-16(14)18/h1-12H,(H,22,23,24)/b17-12-|Technical Data|Appearance: Solid Power|Purity: ≥98% (or refer to the Certificate of Analysis)|Solubility: DMSO : 62.5 mg/mL (132.03 mM; Need ultrasonic)|Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis|Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.|Shelf Life: ≥360 days if stored properly.|Stock Solution Storage: 0 – 4 oC for 1 month or refer to the Certificate of Analysis.|Drug Formulation: To be determined|HS Tariff Code: 382200|How to use|In Vitro:|Pitstop 2 (20-40 μM; 30 min) inhibits the endocytosis of transferrin in J774A.1 macrophages and does not compromise cell viability.Salinomycin Biological Activity Pitstop 2 (20-40 μM; 30 min) does not affect the internalization of cholera toxin B in J774A.Venetoclax MedChemExpress 1 macrophages.PMID:33145757 Pitstop 2 (0.001-100 μM; 6 h) inhibits the mitotic spindle and impairs mitotic progression in HeLa cells. Pitstop 2 (1-30 μM; 24 h) induces apoptosis and inhibits cell growth in dividing cancer cells. Pitstop 2 (1-30 μM; 48 h) does not affect the growth and viability of non-tumourigenic NIH3T3 fibroblasts.|References:|Kumar GA, et, al. Leishmania donovani Internalizes into Host Cells via Caveolin-mediated Endocytosis. Sci Rep. 2019 Sep 2; 9(1): 12636.Smith CM, et, al. Inhibition of clathrin by pitstop 2 activates the spindle assembly checkpoint and induces cell death in dividing HeLa cancer cells. Mol Cancer. 2013 Jan 17; 12:4.Products are for research use only. Not for human use.|