Name :
Recombinant Human NUDT5 Protein (His Tag)
Biological Activity :
Background :
NUDT5 (Nudix Hydrolase 5) is a Protein Coding gene. This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates. ADP-sugar Pyrophosphatase, also known as NUDT5, eliminates toxic nucleotide derivatives from the cell and regulates the levels of important signaling nucleotides and their metabolites. It is widely expressed in the liver, skin, and other tissues. NUDT5 functions as a MutT-related protein and catalyzes the hydrolysis of 8-oxoGDP to 8-oxoGMP, thereby preventing misincorporation of 8-oxoGua into RNA. NUDT5 may play significant roles in regulating the G1-S transition in mammalian cells. It can also hydrolyze other nucleotide sugars with low activity.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
Q9UKK9
NCBI Gene ID :
Synonyms :
Synonyms :
nudix (nucleoside diphosphate linked moiety X)-type motif 5
Amino Acid Sequence :
Molecular Weight :
The recombinant human NUDT5 consists of 234 amino acids and predicts a molecular mass of 26.3 KDa. It migrates as an approximately 35 KDa band in SDS-PAGE under reducing conditions.
Purity :
> 90 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the human NUDT5 (Q9UKK9) (Glu2-Phe219) was expressed with a polyhistidine tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile 50mM Tris, 10% glycerol, pH 8.0.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
hYSAH1 Protein, Human; YSA1 Protein, Human; YSA1H Protein, Human; YSAH1 Protein, Human NUDT5 背景信息 NUDT5 (Nudix Hydrolase 5) is a Protein Coding gene. This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates. ADP-sugar Pyrophosphatase, also known as NUDT5, eliminates toxic nucleotide derivatives from the cell and regulates the levels of important signaling nucleotides and their metabolites. It is widely expressed in the liver, skin, and other tissues. NUDT5 functions as a MutT-related protein and catalyzes the hydrolysis of 8-oxoGDP to 8-oxoGMP, thereby preventing misincorporation of 8-oxoGua into RNA. NUDT5 may play significant roles in regulating the G1-S transition in mammalian cells. It can also hydrolyze other nucleotide sugars with low activity.
References & Citations :
Ishibashi T. et al., 2004, EMBO Rep. 4 (5): 479-8. Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7. Rush J. et al., 2005, Nat Biotechnol. 23 (1): 94-101. Kamiya H. et al., 2009, DNA Repair. 8 (10): 1250-4.
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