Name :
Recombinant Human NKp80/KLRF1 Protein (His Tag), HPLC-verified
Biological Activity :
Background :
NKp80, also known as KLRF1, is an activating homodimeric C-type lectin-like receptor that is expressed on nearly all-natural killer cells and stimulates their cytotoxicity and cytokine release. NKp80 stimulates cytotoxicity upon engagement of its genetically linked ligand: myeloid-specific CTLR activation-induced C-type lectin (AICL). NKp80, but not NKp80 mutated at tyrosine 7 (NKp80/Y7F), is tyrosine phosphorylated. Accordingly, NKp80/Y7F, but not NKp80/Y3F or NKp80/Y37F, failed to induce cytotoxicity. NKp80 phosphopeptides comprising the Hemi-ITAM-like sequence surrounding tyrosine 7 bound Lck- and Syk-family kinases; accordingly, cross-linking of NKp8, but not NKp80/Y7F, induced Syk phosphorylation. Moreover, inhibition of Syk kinase, but not ZAP-7 kinase, impaired cytotoxic responses through NKp80. Atypical residues in the Hemi-ITAM-like motif of NKp80 cause an altered stoichiometry of phosphorylation but did not substantially affect NK cytotoxicity. Altogether, these results show that NKp80 uses an atypical Hemi-ITAM and Syk kinase to trigger cellular cytotoxicity.
Biological Activity :
Recombinant Human NKp80/KLRF1 Protein, His Tag, HPLC-verified（Cat. No. 10984-H07H）was more than 95% and the molecular weight of this protein is around 32.5 kDa verified by SEC-MALS (Routinely tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NCBI Gene ID :
Synonyms :
Synonyms :
killer cell lectin like receptor F1
Amino Acid Sequence :
Molecular Weight :
The recombinant Human KLRF1 consisting of 182 amino acids and has a calculated molecular mass of 21.22 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 39.3 kDa protein in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 32.5 kDa verified by SEC-MALS (Routinely tested).
Purity :
≥95 % as determined by SDS-PAGE；≥ 90 % as determined by SEC-HPLC；≥ 95 % as determined by SEC-MALS(Routinely tested).
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the Human KLRF1 (NP_057607.1) (Leu60-Tyr231) was expressed, with a polyhistidine tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CLEC5C Protein, Human; NKp80 Protein, Human NKp80/KLRF1 背景信息 NKp80, also known as KLRF1, is an activating homodimeric C-type lectin-like receptor that is expressed on nearly all-natural killer cells and stimulates their cytotoxicity and cytokine release. NKp80 stimulates cytotoxicity upon engagement of its genetically linked ligand: myeloid-specific CTLR activation-induced C-type lectin (AICL). NKp80, but not NKp80 mutated at tyrosine 7 (NKp80/Y7F), is tyrosine phosphorylated. Accordingly, NKp80/Y7F, but not NKp80/Y3F or NKp80/Y37F, failed to induce cytotoxicity. NKp80 phosphopeptides comprising the Hemi-ITAM-like sequence surrounding tyrosine 7 bound Lck- and Syk-family kinases; accordingly, cross-linking of NKp8, but not NKp80/Y7F, induced Syk phosphorylation. Moreover, inhibition of Syk kinase, but not ZAP-7 kinase, impaired cytotoxic responses through NKp80. Atypical residues in the Hemi-ITAM-like motif of NKp80 cause an altered stoichiometry of phosphorylation but did not substantially affect NK cytotoxicity. Altogether, these results show that NKp80 uses an atypical Hemi-ITAM and Syk kinase to trigger cellular cytotoxicity.
References & Citations :
Kuttruff S. et al., 2009, Blood. 113 (2): 358-69. Dennehy KM. et al., 2011, J Immunol. 186 (2): 657-61. Roda-Navarro P. et al., 2000, Eur J Immunol. 30 (2): 568-76.
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