Name :
Recombinant Human MST3 Protein (His Tag)
Biological Activity :
Background :
Aberrant STK24 expression was an independent prognostic indicator in lung adenocarcinoma patients. Its dysregulation was associated with its DNA copy number alteration and methylation. STK24/CCM3-regulated exocytosis plays an important role in the protection of kidneys from ischemia-reperfusion injury.
Biological Activity :
The specific activity was determined to be >30 nmol/min/mg using synthetic PKCtide peptide (ERMRPRKRQGSVRRRV) as substrate.
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
Q9Y6E0-2
NCBI Gene ID :
Synonyms :
Synonyms :
serine/threonine kinase 24
Amino Acid Sequence :
Molecular Weight :
The recombinant human STK24 consists of 450 amino acids and predicts a molecular mass of 50.3 kDa. It migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 82 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human STK24 isoform A (Q9Y6E0-2) (Met 1-His 431) was expressed, with a polyhistidine tag at the N-terminus.
Buffer Solution :
Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 0.1mM EGTA, 0.1mM EDTA, 0.25mM DTTPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Synonyms :
HEL-S-95 Protein, Human; MST3 Protein, Human; MST3B Protein, Human; RP11-111L24.5 Protein, Human; STE20 Protein, Human; STK3 Protein, Human MST3 背景信息 Aberrant STK24 expression was an independent prognostic indicator in lung adenocarcinoma patients. Its dysregulation was associated with its DNA copy number alteration and methylation. STK24/CCM3-regulated exocytosis plays an important role in the protection of kidneys from ischemia-reperfusion injury.
References & Citations :
Lu TJ, et al. (2006) Inhibition of cell migration by autophosphorylated mammalian sterile 20-like kinase 3 (MST3) involves paxillin and protein-tyrosine phosphatase-PEST. J Biol Chem. 281(50): 38405-17.Lu TJ, et al. (2005) Zinc ion acts as a cofactor for serine/threonine kinase MST3 and has a distinct role in autophosphorylation of MST3. J Inorg Biochem. 99(6): 1306-13.Schinkmann K, et al. (1997) Cloning and characterization of a human STE20-like protein kinase with unusual cofactor requirements. J Biol Chem. 272 (45): 28695-703.
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