Name :
Recombinant Human MKK6 Protein
Biological Activity :
Background :
Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6, and MKK6, is a protein that belongs to the protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and to a lesser extent in liver or pancreas.
Biological Activity :
Kinase activity untested
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
NP_002749.2
NCBI Gene ID :
Synonyms :
Synonyms :
mitogen-activated protein kinase kinase 6
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant human MKK6 consists of 336 amino acids and predicts a molecular mass of 37.6 KDa. The apparent molecular mass of the protein is approximately 37 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity :
> 90 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human MKK6 (NP_002749.2) (Met 1-Asp334) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Buffer Solution :
Supplied as sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 8.0.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Synonyms :
MAPKK6 Protein, Human; MEK6 Protein, Human; MKK6 Protein, Human; PRKMK6 Protein, Human; SAPKK-3 Protein, Human; SAPKK3 Protein, Human MKK6 背景信息 Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6, and MKK6, is a protein that belongs to the protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and to a lesser extent in liver or pancreas.
References & Citations :
Raingeaud J., et al.,(1996), MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway. Mol. Cell. Biol. 16:1247-1255.Stein B., et al., (1996), Cloning and characterization of MEK6, a novel member of the mitogen-activated protein kinase kinase cascade.J. Biol. Chem. 271:11427-11433.Han J., et al.,(1996), Characterization of the structure and function of a novel MAP kinase kinase (MKK6).J. Biol. Chem. 271:2886-2891.
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