Name :
Recombinant Human MAP3K8 Protein (GST Tag)
Biological Activity :
Background :
Mitogen-activated protein kinase kinase kinase 8, also known as Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2 and MAP3K8, is a cytoplasm protein that belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K8 is expressed in several normal tissues and human tumor-derived cell lines. Isoform 1 of MAP3K8 is activated specifically during the S and G2/M phases of the cell cycle. MAP3K8 is required for TLR4 activation of the MEK/ERK pathway. It can activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p15. MAP3K8 plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. MAP3K8 oncogene linked to human endometrial carcinoma suggesting that it may be another molecule involved in human endometrial cancer. MAP3K8 may also be an important mediator of intracellular mechanotransduction in human bone marrow-derived mesenchymal stem cells (MSCs).
Biological Activity :
Kinase activity untested
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
P41279-1
NCBI Gene ID :
Synonyms :
Synonyms :
mitogen-activated protein kinase kinase kinase 8
Amino Acid Sequence :
Molecular Weight :
The recombinant human MAP3K8/GST chimera consists of 592 amino acids and has a predicted molecular mass of 68 kDa as estimated in SDS-PAGE under reducing conditions.
Purity :
> 91 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human MAP3K8 isoform 1 (P41279-1) (Met 30-Arg 397) was fused with the GST tag at the N-terminus.
Buffer Solution :
Supplied as sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM GSH, pH 8.0Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Synonyms :
AURA2 Protein, Human; c-COT Protein, Human; COT Protein, Human; EST Protein, Human; ESTF Protein, Human; MEKK8 Protein, Human; Tpl-2 Protein, Human; TPL2 Protein, Human MAP3K8 背景信息 Mitogen-activated protein kinase kinase kinase 8, also known as Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2 and MAP3K8, is a cytoplasm protein that belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K8 is expressed in several normal tissues and human tumor-derived cell lines. Isoform 1 of MAP3K8 is activated specifically during the S and G2/M phases of the cell cycle. MAP3K8 is required for TLR4 activation of the MEK/ERK pathway. It can activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p15. MAP3K8 plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. MAP3K8 oncogene linked to human endometrial carcinoma suggesting that it may be another molecule involved in human endometrial cancer. MAP3K8 may also be an important mediator of intracellular mechanotransduction in human bone marrow-derived mesenchymal stem cells (MSCs).
References & Citations :
Clark,A.M. et al., 2004, Genes Chromosomes Cancer. 41 (2):99-108. Chan,H. et al., 2005, Biochem Biophys Res Commun. 328 (1):198-205. Aparecida Alves,C. et al., 2006, Eur J Gynaecol Oncol. 27 (6):589-93. Mielke,L.A. et al., 2009, J Immunol. 183 (12):7984-93. Glossop,J.R. et al., 2009,Gene Expr Patterns 9 (5):381-8.
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C-Type Lectin Domain Family 10 Member A/CD301