Name :
Recombinant Human LY6G6D Protein (His Tag)
Biological Activity :
Background :
The Lymphocyte Antigen 6 Family Member G6D (LY6G6D) is a member of the LY6 family of proteins (typically 70-80 amino acids), mostly GPI-anchored to the cell surface. Regulated by the JAK-STAT5 and RAS-MEK-ERK cascades and belonging to a cluster of leukocyte antigens located in the MHC III region on chromosome 6, LY6G6D demonstrates selective expression in colorectal cancer (CRC), particularly the microsatellite stable subtype, with minimal to no expression in normal tissues. LY6G6D expression is also enhanced in diseases characterized by systemic immune suppression, such as autosomic dominant monocytopenia. LY6G6D up-regulation was predominant in CRCs characterized by an enrichment of immune suppressive regulatory T-cells and limited PD-1/PD-L1 immune checkpoint receptors, demonstrating its potential as a target for novel T-cell-dependent bispecific antibodies that can redirect T-cell effector function to colorectal tumors.
Biological Activity :
Immobilized Anti-ly6g6d human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human LY6G6D Protein (His Tag) (Cat: 24266-H07H), the EC50 is 10-30 ng/mL.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
O95868
NCBI Gene ID :
Synonyms :
Synonyms :
Amino Acid Sequence :
Molecular Weight :
The recombinant Human LY6G6D consists of 94 amino acids and predicts a molecular mass of 10.39 kDa. It migrates as an approximately 23.8 kDa and 20.9 kDa band in SDS-PAGE under reducing conditions.
Purity :
≥ 90 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the Human LY6G6D (O95868) (Asn20-Asn103) was expressed, with a polyhistidine tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
DADB-110M10.6 Protein, Human; G6D Protein, Human; LY6-D Protein, Human; MEGT1 Protein, Human; NG25 Protein, Human LY6G6D 背景信息 The Lymphocyte Antigen 6 Family Member G6D (LY6G6D) is a member of the LY6 family of proteins (typically 70-80 amino acids), mostly GPI-anchored to the cell surface. Regulated by the JAK-STAT5 and RAS-MEK-ERK cascades and belonging to a cluster of leukocyte antigens located in the MHC III region on chromosome 6, LY6G6D demonstrates selective expression in colorectal cancer (CRC), particularly the microsatellite stable subtype, with minimal to no expression in normal tissues. LY6G6D expression is also enhanced in diseases characterized by systemic immune suppression, such as autosomic dominant monocytopenia. LY6G6D up-regulation was predominant in CRCs characterized by an enrichment of immune suppressive regulatory T-cells and limited PD-1/PD-L1 immune checkpoint receptors, demonstrating its potential as a target for novel T-cell-dependent bispecific antibodies that can redirect T-cell effector function to colorectal tumors.
References & Citations :
1. Corrales L, et al. LY6G6D is a selectively expressed colorectal cancer antigen that can be used for targeting a therapeutic T-cell response by a T-cell engager. Front Immunol, 2022. doi: 10.3389/fimmu.2022.1008764. 2. Wang P, et al. Novel Anti-LY6G6D/CD3 T-Cell-Dependent Bispecific Antibody for the Treatment of Colorectal Cancer. Mol Cancer Ther, 2022. doi: 10.1158/1535-71633. Giordano G, et al. JAK/Stat5-mediated subtype-specific lymphocyte antigen 6 complex, locus G6D (LY6G6D) expression drives mismatch repair proficient colorectal cancer. J Exp Clin Cancer Res, 2019. doi: 10.1186/s13046-018-1019-5
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