Name :
Recombinant Human LRP-6 Protein (His & AVI Tag), Biotinylated, HPLC-verified
Biological Activity :
Background :
Lipoprotein receptor-related protein 6 (LRP6) binds to Wnt ligands to transduce signal by stabilization of beta-catenin, which has been involved in the regulation of embryonic development and metabolism. Low density lipoprotein (LDL) receptor-related protein-6 (LRP6) is an important co-receptor of Wnt pathway, which plays a predominant role in the development and progression of colorectal cancer. This gene, termed LDL receptor-related protein 6 (LRP6), encodes a transmembrane protein that has 71% identity and is structurally similar to the protein encoded by LRP5, a proposed candidate gene for type 1 diabetes located on human chromosome 11q13. LRP6 maps to human chromosome 12p11-p13.
Biological Activity :
1.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 11968-H49H-B) on SA Biosensor, can bind Recombinant Human DKK-1 Protein, His Tag (Cat. No. 10170-H08H) with an affinity constant of 90.6 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).2.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 11968-H49H-B) on SA Biosensor, can bind Recombinant Human R-Spondin 1/RSPO1 Protein, His Tag (Cat. No. 11083-H08H) with an affinity constant of 0.983 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested).3.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 11968-H49H-B) on SA Biosensor, can bind Recombinant Human SOST/Sclerostin Protein, His Tag (Cat. No. 10593-H07H) with an affinity constant of 39.5 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).4.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 11968-H49H-B) on SA Biosensor, can bind Recombinant Human DKK-1 Protein, hFc Tag (Cat. No. 10170-H02H) with an affinity constant of 54.3 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
O75581
NCBI Gene ID :
Synonyms :
Synonyms :
LDL receptor related protein 6
Amino Acid Sequence :
Molecular Weight :
The recombinant Human LRP6 consists of 637 amino acids and predicts a molecular mass of 72.22 kDa. It migrates as an approximately 79.8 kDa band in SDS-PAGE under reducing conditions.
Purity :
≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the Human LRP6 (O75581) (Met1-Pro630) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
ADCAD2 Protein, Human LRP-6 背景信息 Lipoprotein receptor-related protein 6 (LRP6) binds to Wnt ligands to transduce signal by stabilization of beta-catenin, which has been involved in the regulation of embryonic development and metabolism. Low density lipoprotein (LDL) receptor-related protein-6 (LRP6) is an important co-receptor of Wnt pathway, which plays a predominant role in the development and progression of colorectal cancer. This gene, termed LDL receptor-related protein 6 (LRP6), encodes a transmembrane protein that has 71% identity and is structurally similar to the protein encoded by LRP5, a proposed candidate gene for type 1 diabetes located on human chromosome 11q13. LRP6 maps to human chromosome 12p11-p13.
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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