Name :
Recombinant Human LAG-3 Protein (ECD), HPLC-verified
Biological Activity :
Background :
LAG3 (Lymphocyte Activating 3) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. LAG3, also known as CD223 and Lymphocyte activation gene 3, belongs to the immunoglobulin (Ig) superfamily. The LAG3 gene contains 8 exons. It is selectively expressed in activated T and NK cells. LAG3 contains 4 extracellular Ig-like domains and has a negative regulatory function in T cells. It also acts as a new marker of T cell-induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signaling, leading to increased antigen-specific T-cell responses in vivo. Diseases associated with LAG3 include Smoldering Myeloma and Kyphoscoliotic Heart Disease. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Targets Immunotherapy Targeted Therapy
Biological Activity :
Immobilized Recombinant Human LAG3 / CD223 / Lymphocyte activation gene 3 Protein (ECD) Cat: 16498-HNAH) at 2μg/mL (100μL/well) can bind Anti-LAG3(BMS)-IgG4 Antibody, the EC50 is 45-130 ng/mL.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_002277.4
NCBI Gene ID :
Synonyms :
Synonyms :
lymphocyte-activation gene 3
Amino Acid Sequence :
Molecular Weight :
The recombinant human LAG3 consists of 418 amino acids and predicts a molecular mass of 45.3 kDa. As a result of glycosylation, it migrates as an approximately 56.1 KDa band in SDS-PAGE under reducing conditions.
Purity :
> 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the human LAG3 (NP_002277.4) (Met1-Arg440) was expressed.
Buffer Solution :
Lyophilized from sterile PBS,pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CD223 Protein, Human; LAG-3 Protein, Human LAG-3 背景信息 LAG3 (Lymphocyte Activating 3) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. LAG3, also known as CD223 and Lymphocyte activation gene 3, belongs to the immunoglobulin (Ig) superfamily. The LAG3 gene contains 8 exons. It is selectively expressed in activated T and NK cells. LAG3 contains 4 extracellular Ig-like domains and has a negative regulatory function in T cells. It also acts as a new marker of T cell-induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signaling, leading to increased antigen-specific T-cell responses in vivo. Diseases associated with LAG3 include Smoldering Myeloma and Kyphoscoliotic Heart Disease. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Targets Immunotherapy Targeted Therapy
References & Citations :
Sigrid Hannier. et al., 1998, The Journal of Immunology. 161(8): 4058-65. Triebel F. et al., 1990, J Exp Med. 171 (5): 1393-405. Baixeras E. et al., 1992, J Exp Med. 176 (2): 327-37. Huard B. et al., 1997, Proc Natl Acad Sci. 94 (11): 5744-9. Kisielow M. et al., 2005, Eur J Immunol. 35 (7): 2081-8.
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