Name :
Recombinant Human ITPRIPL1 Protein (ECD, His Tag), HPLC-verified

Biological Activity :

Background :
Inositol 1,4,5-trisphosphate Receptor Interacting Protein-Like 1 or ITPRIPL1 is a transmembrane protein with broad expression in the testis associated with immune evasion. In fact, ITPRIPL1 is a molecule specifically expressed in immune privileged tissues, “hot” tumors without PD-L1 expression, and non-responding tumors to PD-1 blockade therapy. ITPRIPL1 has been shown to impair T cell function in vitro and in vivo through CD3e with experimental evidence supporting a negative correlation between ITPRIPL1 expression level in antigen-presenting cells and T cell cytotoxicity. ITPRIPL1 was a hypermethylated-low expression gene in breast cancer and acted as an independent biomarker for the prognosis of lung adenocarcinoma by using bioinformatics methods. Thus, targeting the ITPRIPL1-CD3e axis, especially in PD-1 – PD-L1 negative patients, is a promising therapeutic strategy to reduce immune evasion.

Biological Activity :
Testing in progress

Expression Host :
Human

Source :
HEK293 Cells

Tag :

Protein Accession No. :
AAH34503.2

NCBI Gene ID :

Synonyms :

Synonyms :
inositol 1,4,5-trisphosphate receptor interacting protein-like 1

Amino Acid Sequence :

Molecular Weight :
The recombinant Human ITPRIPL1 consists of 90 amino acids and predicts a molecular mass of 10.75 kDa. It migrates as an approximately 13.2 kDa band in SDS-PAGE under reducing conditions.

Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

State of Matter :

Product Concentration :

Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.

Protein Construction :
A DNA sequence encoding the Human ITPRIPL1 (AAH34503.2) (His33-Gly111) was expressed with a polyhistidine tag at the C-terminus.

Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.

Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Synonyms :
KIAA1754L Protein, Human ITPRIPL1 背景信息 Inositol 1,4,5-trisphosphate Receptor Interacting Protein-Like 1 or ITPRIPL1 is a transmembrane protein with broad expression in the testis associated with immune evasion. In fact, ITPRIPL1 is a molecule specifically expressed in immune privileged tissues, “hot” tumors without PD-L1 expression, and non-responding tumors to PD-1 blockade therapy. ITPRIPL1 has been shown to impair T cell function in vitro and in vivo through CD3e with experimental evidence supporting a negative correlation between ITPRIPL1 expression level in antigen-presenting cells and T cell cytotoxicity. ITPRIPL1 was a hypermethylated-low expression gene in breast cancer and acted as an independent biomarker for the prognosis of lung adenocarcinoma by using bioinformatics methods. Thus, targeting the ITPRIPL1-CD3e axis, especially in PD-1 – PD-L1 negative patients, is a promising therapeutic strategy to reduce immune evasion.

References & Citations :
1. Shouyan Deng, et al. The ITPRIPL1- CD3ε axis: a novel immune checkpoint controlling T cells activation. bioRxiv, 2022. doi: 10.1101/2022.02.25.4811892. Zhong, X. and Zhong, G. Prognostic biomarker identification and tumor classification in breast cancer patients by methylation and transcriptome analysis. FEBS Open Bio, 2021. doi: 10.1002/2211-5463.13211

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