Name :
Human INS Protein
Biological Activity :
Background :
INS (Insulin) is a Protein Coding gene. This gene encodes Insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proInsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form Insulin, and C-peptide. The binding of Insulin to the Insulin receptor (INSR) stimulates glucose uptake. Diseases associated with INS include HyperproInsulinemia and Maturity-Onset Diabetes Of The Young, Type 10. A multitude of mutant alleles with phenotypic effects has been identified, including Insulin-dependent diabetes mellitus, permanent neonatal diabetes mellitus, maturity-onset diabetes of the young type 10, and hyperproInsulinemia. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy
Biological Activity :
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells.The ED50 for this effect is typically 0.2-1 µg/mL.
Expression Host :
Human
Source :
Yeast
Tag :
Protein Accession No. :
P01308
NCBI Gene ID :
Synonyms :
Synonyms :
Insulin
Amino Acid Sequence :
Molecular Weight :
The recombinant human Insulin consists of 63 amino acids and predicts a molecular mass of 7.1 KDa. It migrates as an approximately 6 KDa band in SDS-PAGE under reducing conditions.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding human Insulin B chain (P01308)(Phe25-Lys53) and human Insulin A chain (P01308)(Gly90-Asn110) was expressed with a linker AAK.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
IDDM Protein, Human; IDDM1 Protein, Human; IDDM2 Protein, Human; ILPR Protein, Human; INS Protein, Human; IRDN Protein, Human; MODY10 Protein, Human Insulin 背景信息 INS (Insulin) is a Protein Coding gene. This gene encodes Insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proInsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form Insulin, and C-peptide. The binding of Insulin to the Insulin receptor (INSR) stimulates glucose uptake. Diseases associated with INS include HyperproInsulinemia and Maturity-Onset Diabetes Of The Young, Type 10. A multitude of mutant alleles with phenotypic effects has been identified, including Insulin-dependent diabetes mellitus, permanent neonatal diabetes mellitus, maturity-onset diabetes of the young type 10, and hyperproInsulinemia. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy
References & Citations :
Veedfald S, Plamboeck A, Deacon C F, et al. Cephalic phase secretion of Insulin and other enteropancreatic hormones in humans[J]. American Journal of Physiology-Gastrointestinal and Liver Physiology, 2016, 310(1): G43-G51.
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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