Name :
Recombinant Human IL-23 Protein (His & AVI Tag), Biotinylated
Biological Activity :
Background :
Biological Activity :
Immobilized Human IL23 Receptor Protein (ECD, hFc Tag) (Cat:13840-H02H) at 2 μg/mL (100 μL/well) can bind Human IL-23 Protein (His & AVI Tag), Biotinylated (Cat:CT048-H27H-B), the EC50 is 180-540 ng/mL.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_002178.2 & NP_057668.1
NCBI Gene ID :
Synonyms :
Synonyms :
Amino Acid Sequence :
Molecular Weight :
The recombinant human IL23 complex (IL23A&IL12B) consists of 517 amino acids and predicts a molecular mass of 57.6 kDa.
Purity :
> 95 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the human IL23 complex composed of human IL12B (NP_002178.2) (Met1-Ser328) and human IL23A (NP_057668.1) (Arg20-Pro189) linked by a polypeptide linker was fused with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Buffer Solution :
Supplied as sterile 50 mM Tris, 100 mM Nacl, 10%glycerol, 0.02% Tween 80, 10% Trehalose, pH 8.5.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Liquid. It is shipped out with blue ice.
Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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