Name :
Recombinant Human HK3 Protein (His & GST Tag)
Biological Activity :
Background :
Hexokinase-3, also known as Hexokinase type III, HKIII, and HK3, is a protein that belongs to the hexokinase family. Hexokinase-3 / HK3 is an enzyme that in humans is encoded by the HK2 gene. Hexokinases phosphorylate glucose to produce glucose 6-phosphate, committing glucose to the glycolytic pathway. In mammalian tissues, hexokinase exists as four isoenzymes encoded by distinct genes. These proteins are homologous and are organized in two homologous domains, except for hexokinase type IV which has only one. This organization is believed to be the result of a duplication and tandem fusion event involving the gene encoding for the ancestral hexokinase. The gene encodes hexokinase-3. Similar to hexokinases-1 and hexokinases-2, this allosteric enzyme is inhibited by its product glucose 6-phosphate.
Biological Activity :
Kinase activity untested
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
P52790
NCBI Gene ID :
Synonyms :
Synonyms :
hexokinase 3 (white cell)
Amino Acid Sequence :
Molecular Weight :
The recombinant human HK3/GST chimera consists of 1160 amino acids and has a calculated molecular mass of 127 kDa. It migrates as an approximately 115 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 85 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human HK3 (P52790) (Met 1-Val 923) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Buffer Solution :
Supplied as sterile 50mM Tris, 100mM Nacl, pH7.4, 25% glycerol, 0.5mM PMSF, 0.1mM EDTA, 0.5mM GSHPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Synonyms :
HKIII Protein, Human; HXK3 Protein, Human HK3 背景信息 Hexokinase-3, also known as Hexokinase type III, HKIII, and HK3, is a protein that belongs to the hexokinase family. Hexokinase-3 / HK3 is an enzyme that in humans is encoded by the HK2 gene. Hexokinases phosphorylate glucose to produce glucose 6-phosphate, committing glucose to the glycolytic pathway. In mammalian tissues, hexokinase exists as four isoenzymes encoded by distinct genes. These proteins are homologous and are organized in two homologous domains, except for hexokinase type IV which has only one. This organization is believed to be the result of a duplication and tandem fusion event involving the gene encoding for the ancestral hexokinase. The gene encodes hexokinase-3. Similar to hexokinases-1 and hexokinases-2, this allosteric enzyme is inhibited by its product glucose 6-phosphate.
References & Citations :
Palma F. et al., 1996, Mol Cell Biochem. 155: 23-9. Furuta H. et al.,1996, Genomics. 36 (1): 206-9. Colosimo A. et al.,1996, Cytogenet Cell Genet. 74 (3): 187-8.
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