Name :
Recombinant Human, Mouse HIST1H3A Protein
Biological Activity :
Background :
Histone H3.1, also known as HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, is a member of the histone H3 family which is a core component of nucleosome. It is expressed during the S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher-order chromatin structures.
Biological Activity :
Testing in progress
Expression Host :
Human, Mouse
Source :
E. coli
Tag :
Protein Accession No. :
NP_003520.1
NCBI Gene ID :
Synonyms :
Synonyms :
histone cluster 1, H3a
Amino Acid Sequence :
Molecular Weight :
The recombinant human / mouse HIST1H3A consisting of 136 amino acids and has a predicted molecular mass of 15.5 kDa as estimated in SDS-PAGE under reducing conditions.
Purity :
> 95 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the native human / mouse HIST1H3A (NP_003520.1) (Met1-Ala136) was expressed. Human and Mouse HIST1H3A sequences are identical.
Buffer Solution :
Lyophilized from sterile 2mM β-MercaptoethanolPlease contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
References & Citations :
Lachner M, et al., 2001, Nature 410 (6824): 116-20. Koessler H, et al., 2003, DNA Cell Biol. 22 (4): 233-41. Macdonald N. et al., 2005, Mol. Cell 20: 199-211. Hyllus D. et al., 2007, Genes Dev. 21: 3369-3380. Garcia BA. et al., 2007, J. Biol. Chem. 282:7641-7655. Yu L.-R. et al., 2007, J. Proteome Res. 6: 4150-4162.
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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